Anne L Powell

US Patent:

58664203, Feb 2, 1999

Filed:

Jul 1, 1996

Appl. No.:

8/674068

Inventors:

Neil C. Talbot - Silver Spring MD
Christopher W. Kemp - Frederick MD
Thomas J. Caperna - Arnold MD
Caird E. Rexroad - Gambrills MD
Vernon G. Pursel - Highland MD
Anne M. Powell - Bowie MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 500

US Classification:

435395

Abstract:

Continuous cultures of pluripotent parenchymal hepatocytes were derived from the epiblasts of pig blastocysts. The cultures are feeder-dependent and grow slowly with doubling times of 3 to 4 days. They differentiate into large secretory duct-like structures or form small canaliculi. In combination with feeder cells and, optionally, adult pig hepatocytes and macrophages, the cells are useful in an artificial liver device which may be utilized as temporary liver support for the mitigation of the pathological effects of liver failure.

US Patent:

55321569, Jul 2, 1996

Filed:

Oct 8, 1993

Appl. No.:

8/133589

Inventors:

Neil Talbot - Silver Spring MD
Caird E. Rexroad - Gambrills MD
Vernon G. Pursel - Highland MD
Anne M. Powell - Bowie MD

Assignee:

The United States of America as represented by the Secretary of
Agriculture - Washington DC

International Classification:

C12N 500

US Classification:

4352402

Abstract:

Continuous cultures of pluripotent parenchymal hepatocytes were derived from the epiblasts of pig blastocysts. The cultures are feeder-dependent and grow slowly with doubling times of 3 to 4 days. They differentiate into large secretory duct-like structures or form small canaliculi. Alternatively, the cells accumulate droplets that stain intensely with oil red O, a lipid-specific stain. . alpha. -Fetoprotein and albumin mRNA expression increases as the cells differentiate in culture.