Brian Isadore Root

US Patent:

20180304253, Oct 25, 2018

Filed:

Oct 13, 2016

Appl. No.:

15/768115

Inventors:

- Charlottesville VA, US
Jacquelyn A. DuVall - Raleigh NC, US
Delphine Le Roux - Barboursville VA, US
Brian Root - Charlottesville VA, US
Daniel MIlls - Charlottesville VA, US
Daniel A. Nelson - Charlottesville VA, US
An-chi Tsuei - Charlottesville VA, US
Brandon L. Thompson - Charlottesville VA, US
Jingyi Li - Charlottesville VA, US
Christopher Birch - Charlottesville VA, US

International Classification:

B01L 3/00
B01L 7/00
G01N 27/447

Abstract:

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.