Brian K Washburn

US Patent:

7364854, Apr 29, 2008

Filed:

Jan 21, 2005

Appl. No.:

11/040371

Inventors:

Brian Washburn - Durham NC, US
Douglas Lovern - Durham NC, US
Hwa-Tang Thomas Wang - Encinitas CA, US
Lawrence Burg - Framingham MA, US

Assignee:

bioMerieux, Inc - Durham NC

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435 6, 435 911, 435 912

Abstract:

The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates.

US Patent:

7670779, Mar 2, 2010

Filed:

Feb 11, 2008

Appl. No.:

12/069606

Inventors:

Brian Washburn - Durham NC, US
Douglas Lovern - Durham NC, US
Hwa-Tang Thomas Wang - Encinitas CA, US
Lawrence Burg - Framingham MA, US

Assignee:

bioMerieux, Inc. - Durham NC

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification. In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates.

US Patent:

20060003343, Jan 5, 2006

Filed:

Jan 21, 2005

Appl. No.:

11/040417

Inventors:

Hwa-Tang Wang - Encinitas CA, US
Brian Washburn - Durham NC, US
Prakash Purohit - Shrewsbury MA, US
Lawrence Burg - Framingham MA, US

International Classification:

C12Q 1/68
C12N 5/06
C12N 5/16

US Classification:

435006000, 435345000

Abstract:

The present invention provides selected oligonucleotides corresponding to portions of the 3′ non-translating region of Hepatitis C virus. The invention also includes methods of detecting and of quantitating HCV nucleic acids in a sample using these oligonucleotides.

US Patent:

20110245094, Oct 6, 2011

Filed:

May 1, 2009

Appl. No.:

12/990761

Inventors:

Brian K. Washburn - Boston MA, US
Andrew E. Levin - Boston MA, US

Assignee:

IMMUNETICS, INC. - Boston MA

International Classification:

C40B 30/04
C12N 1/15
C07H 21/04
C40B 40/06

US Classification:

506 9, 43525411, 536 231, 536 2433, 506 16

Abstract:

The present invention features, inter alia, compositions and methods useful for identifying one or more types of microorganisms, if and when present, in a sample or plurality of samples (e.g., in one or more samples tested in parallel). More specifically, the present compositions and methods can be used in, e.g., determining whether a subject has a microbial infection (e.g., a bacterial, fungal, protozoal, or viral infection), determining the identity of the microbe(s) causing the infection, and/or determining, or helping to determine, an appropriate anti-microbial treatment regimen for a subject identified as having an infection (e.g., an appropriate antibiotic, anti-fungal, anti-viral, or other treatment regimen).