Bruce E Eagan

US Patent:

7172884, Feb 6, 2007

Filed:

Sep 22, 2003

Appl. No.:

10/668032

Inventors:

Daniel Benigni - Skaneateles NY, US
Robert Stankavage - Bridgeport NY, US
Shu-Jen Chiang - Manlius NY, US
Hsing Hou - Manlius NY, US
Bruce Eagan - Fayetteville NY, US
Dennis Gu - Skokie IL, US
David Hou - Baldwinsville NY, US
Les Mintzmyer - Manlius NY, US
Thomas P. Tully - Middlesex NJ, US
Brian L. Davis - Milltown NJ, US
Ivan Hargro - Roselle NJ, US
Mark Mascari - East Syracuse NY, US
Gabriel Galvin - Manlius NY, US
Gregory Stein - Mallory NY, US
Cary W. McConlogue - Plainsboro NJ, US
Fahri T. Comezoglu - Kendall Park NJ, US

Assignee:

Bristol-Myers Squibb Company - Princeton NJ

International Classification:

C08B 30/18
A61K 31/724
A61K 31/715
C12P 17/18

US Classification:

435118, 435120, 514 58, 514365, 536 46

Abstract:

The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

US Patent:

20070122891, May 31, 2007

Filed:

Jan 31, 2007

Appl. No.:

11/669268

Inventors:

Daniel Benigni - Skaneateles NY, US
Robert Stankavage - Bridgeport NY, US
Shu-Jen Chiang - Manlius NY, US
Hsing Hou - Manlius NY, US
Bruce Eagan - Fayetteville NY, US
Dennis Gu - Lake Bluff IL, US
David Hou - San Ramon CA, US
Les Mintzmyer - Manlius NY, US
Thomas Tully - Middlesex NJ, US
Brian Davis - Milltown NJ, US
Ivan Hargro - Plainfield NJ, US
Mark Mascari - East Syracuse NY, US
Gabriel Galvin - Manlius NY, US
Gregory Stein - Mallory NY, US
Cary McConlogue - Plainsboro NJ, US
Fahri Comezoglu - Kendall Park NJ, US

International Classification:

C12P 17/00
C12N 1/20
C07D 417/02

US Classification:

435117000, 435252300, 548181000

Abstract:

The present invention relates to improved methods for the production, isolation and purification of epothilone B. These methods include, for example, a fermentation process for the production of epothilone B, isolation via adsorption onto a resin, and subsequent purification.

US Patent:

20160024203, Jan 28, 2016

Filed:

Mar 14, 2014

Appl. No.:

14/774707

Inventors:

- Princeton NJ, US
George S. Campbell - Fayetteville NY, US
Bruce E. Eagan - Dewitt NY, US
Yueming Qian - Manlius NY, US
Xuankuo Xu - Manlius NY, US
Li You - Jamesville NY, US
Zhengjian Li - Sudbury MA, US
Nan-Xin Qian - Manlius NY, US

International Classification:

C07K 16/24

Abstract:

The present invention describes a method for producing an antibody in , such as by fed-batch fermentation. The method includes the addition of about 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may also include a strategy of increasing the ethanol concentration to about 18-22 g/L and then maintaining the ethanol level at about 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.

US Patent:

20160024204, Jan 28, 2016

Filed:

Mar 14, 2014

Appl. No.:

14/774709

Inventors:

- Princeton NJ, US
George S. Campbell - Fayetteville NY, US
Bruce E. Eagan - Dewitt NY, US
Yueming Qian - Manlius NY, US
Xuankuo Xu - Manlius NY, US
Li You - Jamesville NY, US
Zhengjian Li - Sudbury MA, US
Nan-Xin Qian - Manlius NY, US

International Classification:

C07K 16/24

Abstract:

The present invention describes a method for producing an antibody in , such as by fed-batch fermentation. The method includes a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain. The method may also include a strategy of increasing the ethanol concentration to about 18-22 g/L and then maintaining the ethanol level at about 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may also include the addition of about 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody.

US Patent:

20140273093, Sep 18, 2014

Filed:

Mar 14, 2014

Appl. No.:

14/210508

Inventors:

- Princeton NJ, US
George S. Campbell - Fayetteville NY, US
Bruce E. Eagan - Dewitt NY, US
Yueming Qian - Manlius NY, US
Xuankuo Xu - Manilus NY, US
Li You - Jamesville NY, US
Zhengjian Li - Jamesville NY, US
Nan-Xin Qian - Manlius NY, US

Assignee:

BRISTOL-MYERS SQUIBB COMPANY - Princeton NJ

International Classification:

C07K 16/24

US Classification:

435 696

Abstract:

The present invention describes a method for producing an antibody in such as by fed-batch fermentation. The method may include a strategy of increasing the ethanol concentration to 18-22 g/L and then maintaining the ethanol level at 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may also include the addition of 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.

US Patent:

20140273094, Sep 18, 2014

Filed:

Mar 14, 2014

Appl. No.:

14/210573

Inventors:

- PRINCETON NJ, US
GEORGE S. CAMPBELL - FAYETTEVILLE NY, US
BRUCE E. EAGAN - DEWITT NY, US
YUEMING QIAN - MANLIUS NY, US
XUANKUO XU - MANLIUS NY, US
LI YOU - JAMESVILLE NY, US
ZHENGJIAN LI - JAMESVILLE NY, US
NAN-XIN QIAN - MANLIUS NY, US

Assignee:

BRISTOL-MYERS SQUIBB COMPANY - PRINCETON NJ

International Classification:

C07K 16/24

US Classification:

435 696

Abstract:

The present invention describes a method for producing an antibody in , such as by fed-batch fermentation. The method may include a strategy of increasing the ethanol concentration to 18-22 g/L and then maintaining the ethanol level at 5-17 g/L to stabilize the cell mass and enhance the production rate of the antibody. The method may also include the addition of 2.0-5.0 g/L of hydroxyurea during the fermentation process to sustain a constant cell density and enhance the whole broth titer of the antibody. The method may further include a respiratory quotient control for monitoring the ethanol profile and to improve the quality of the antibody by, for example, eliminating clipping of the heavy chain.