Carl E Fuller

US Patent:

6875573, Apr 5, 2005

Filed:

Jul 8, 2002

Appl. No.:

10/190967

Inventors:

Carl Fuller - Berkeley Heights NJ, US
Joseph Szasz - Chardon OH, US

Assignee:

Amersham Biosciences Corp - Piscataway NJ

International Classification:

C12Q001/68
C07H021/04

US Classification:

435 6, 536 231, 536 243, 536 2433, 530350

Abstract:

Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.

US Patent:

7223541, May 29, 2007

Filed:

Feb 5, 2003

Appl. No.:

10/358818

Inventors:

Carl Fuller - Berkeley Heights NJ, US
Shiv Kumar - Belle Mead NJ, US
Anup Sood - Flemington NJ, US
John Nelson - Hillsborough NJ, US

Assignee:

GE Healthcare Bio-Sciences Corp. - Piscataway NJ

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

The present invention relates to improved methods of detecting a target using a labeled substrate or substrate analog. The methods comprise reacting the substrate or substrate analog in an enzyme-catalyzed reaction which produces a labeled moiety with independently detectable signal only when such substrate or substrate analog reacts. The present invention, in particular, describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon.

US Patent:

7264934, Sep 4, 2007

Filed:

Oct 21, 2005

Appl. No.:

11/255683

Inventors:

Carl W. Fuller - Berkeley Heights NJ, US

Assignee:

GE Healthcare Bio-Sciences Corp. - Piscataway NJ

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243

Abstract:

This invention provides methods for massive parallel nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the nucleotide complementary to the next template nucleotide in the closed complex. Detection of the trapped nucleotide allows determination of the sequence of this next correct nucleotide. In this way, sequential nucleotides of a nucleic acid template can be identified, effectively determining the sequence. This method is applied to sequence multiple templates in parallel, particularly if they are immobilized on a solid support.

US Patent:

7402654, Jul 22, 2008

Filed:

Dec 22, 2005

Appl. No.:

11/315390

Inventors:

Carl W. Fuller - Berkeley Heights NJ, US
Cuong Lam - Piscataway NJ, US

Assignee:

GE Healthcare Bio-Sciences Corp. - Piscataway NJ

International Classification:

A61K 38/00
C12Q 1/68
C07K 1/00

US Classification:

530324, 514 2, 530350, 435 6

Abstract:

A purified recombinant DNA polymerase having high processivity and strand-displacement activity. An isolated nucleic acid that encodes the bacteriophage DNA polymerase. A kit and method for amplifying DNA is also disclosed.

US Patent:

7871771, Jan 18, 2011

Filed:

Jun 9, 2005

Appl. No.:

11/570347

Inventors:

Carl W. Fuller - Berkeley Heights NJ, US
John R. Nelson - Clifton Park NY, US

Assignee:

GE Healthcare Bio-Sciences Corp. - Piscataway NJ

International Classification:

C12Q 1/68
C07H 21/02
C07H 21/04

US Classification:

435 6, 536 231, 536 243

Abstract:

This invention provides methods for nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the labeled nucleotide complementary to the next template nucleotide in the closed complex. Detection of the label allows determination of the identity of this next correct nucleotide. Identification can be either in place, as part of the complex, or as the dye is eluted from the complex when the reaction cycle is completed by the addition of divalent metal ion. In this way, sequential nucleotides of a DNA can be identified, effectively determining the DNA sequence. This method can be applied to nucleic acid single molecules or to collections of identical or nearly identical sequence such as PCR products or clones. Multiple templates can be sequenced in parallel, particularly if they are immobilized on a solid support.

US Patent:

52122948, May 18, 1993

Filed:

Mar 31, 1992

Appl. No.:

7/860938

Inventors:

John E. Starrett - Middletown CT
Muzammil M. Mansuri - Cheshire CT
John C. Martin - Cheshire CT
Carl E. Fuller - Warners NY
Henry G. Howell - Jamesville NY

Assignee:

Bristol-Myers Squibb Company - New York NY

International Classification:

C07H 1700
C07H 1906

US Classification:

536 282

Abstract:

There are disclosed novel processes for producing 2',3'-dideoxy-2'3'-didehydronucleosides, for example, 2',3'-dideoxy-2',3'-didehydrothymidine in high yields and on a large scale. The compounds so-produced are useful as antiviral agents, especially as agents effective against the human immunodeficiency viruses (HIV).

US Patent:

49047701, Feb 27, 1990

Filed:

Mar 24, 1988

Appl. No.:

7/173473

Inventors:

John E. Starrett - Middletown CT
Muzammil M. Mansuri - Cheshire CT
John C. Martin - Cheshire CT
Carl E. Fuller - Warners NY
Henry G. Howell - Jamesville NY

Assignee:

Bristol-Myers Company - New York NY

International Classification:

C07H 1906

US Classification:

536 23

Abstract:

There are disclosed novel processes for producing 2',3'-dideoxy-2'3'-didehydronucleosides, for example, 2',3'-dideoxy-2',3'-didehydrothymidine in high yields and on a large scale. The compounds so-produced are useful as antiviral agents, especially as agents effective against the human immunodeficiency viruses (HIV).

US Patent:

51304210, Jul 14, 1992

Filed:

Apr 29, 1991

Appl. No.:

7/697512

Inventors:

John E. Starrett - Middletown CT
Muzammil M. Mansuri - Cheshire CT
John C. Martin - Cheshire CT
Carl E. Fuller - Warners NY
Henry G. Howell - Jamesville NY

Assignee:

Bristol-Myers Company - New York NY

International Classification:

C07H 1700

US Classification:

536 23

Abstract:

There are disclosed novel processes for producing 2',3'-dideoxy-2'3'-didehydronucleosides, for example, 2',3'-dideoxy-2',3'-didehydrothymidine in high yields and on a large scale. The compounds so-produced are useful as antiviral agents, especially as agents effective against the human immunodeficiency viruses (HIV).