Dawn D Newman

US Patent:

46346783, Jan 6, 1987

Filed:

Dec 13, 1982

Appl. No.:

6/449187

Inventors:

John S. Salstrom - Edina MN
Dawn Newman - Hopkins MN
Douglas F. Harbrecht - Hopkins MN
Shiu-Lok Hu - Minnetonka MN

Assignee:

Molecular Genetics Research and Development Limited Partnership - Minnetonka MN

International Classification:

C12N 100
C12N 1500
C12N 120
C12P 2100
C12P 2102
C12P 1934

US Classification:

435317

Abstract:

DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment which provides a means for inserting nucleic acid sequences and (d) a sequence coding for a detectable gene product, out of translational phase with the translation start codon but capable of being readjusted to the translational phase of said start codon by insertion into the cloning segment of nucleic acid sequences containing the proper number of nucleotides for readjustment, said gene product providing a means for detecting expression of inserted nucleic acid sequences.