Frances W Arnold

US Patent:

40917657, May 30, 1978

Filed:

Feb 17, 1977

Appl. No.:

5/769441

Inventors:

Ernest C. Lowthorp - late of Stone Mountain GA
Frances T. Arnold - Atlanta GA
Luther R. Winters - Orlando FL
Roy Lippert - Orlando FL

Assignee:

Vivian L. - Stone Mountain GA

International Classification:

G03G 1509

US Classification:

118658

Abstract:

A developing and fusing apparatus for applying a single component magnetically responsive dry particulate toner powder to a latent electrostatic image on electrostatic copy paper and fusing the thusly applied toner powder onto the surface of the copy paper with the developing section of the apparatus having a permanent magnet applicator roll assembly which moves the toner powder about the periphery thereof into the vicinity of the copy paper to transfer the toner powder onto the copy paper with the amount of toner powder passing around the applicator roll assembly being controlled by doctor blades, and a cartridge which dispenses the toner powder onto the applicator roll assembly for passage around the applicator roll assembly. The fusing section of the apparatus includes a pair of fusing rolls which are urged toward each other with sufficient force to pressure fuse the toner powder onto the surface of the copy paper along with means for limiting the movement of the fuser rolls toward each other to prevent the fusing roll surfaces from actually contacing each other.

US Patent:

20190211367, Jul 11, 2019

Filed:

Jan 11, 2019

Appl. No.:

16/246246

Inventors:

- Pasadena CA, US
Eric M. Burstad - Durham NC, US
Frances H. Arnold - La Canada CA, US
Zhan Wang - San Jose CA, US
Jared C. Lewis - Chicago IL, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12P 13/02
C12N 9/02
C12P 7/62

Abstract:

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

US Patent:

20170247725, Aug 31, 2017

Filed:

Sep 28, 2016

Appl. No.:

15/278561

Inventors:

- Pasadena CA, US
Eric M. Brustad - Durham NC, US
Frances H. Arnold - La Canada CA, US
Zhan Wang - San Jose CA, US
Jared C. Lewis - Chicago IL, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12P 13/02
C12N 9/02
C12P 7/62

Abstract:

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

US Patent:

20160002682, Jan 7, 2016

Filed:

Feb 18, 2015

Appl. No.:

14/625449

Inventors:

- Pasadena CA, US
Eric M. Brustad - Durham NC, US
Frances H. Arnold - La Canada CA, US
Zhan Wang - San Jose CA, US
Jared C. Lewis - Chicago IL, US

International Classification:

C12P 13/02
C12P 7/62

Abstract:

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

US Patent:

20140242647, Aug 28, 2014

Filed:

Feb 20, 2014

Appl. No.:

14/185861

Inventors:

- Pasadena CA, US
Eric M. Brustad - Durham NC, US
Frances H. Arnold - La Canada CA, US
Zhan Wang - San Jose CA, US
Jared C. Lewis - Chicago IL, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12P 13/02
C12P 7/62

US Classification:

435129, 435189, 435135

Abstract:

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.