Jian Bin Cao

US Patent:

6770446, Aug 3, 2004

Filed:

Apr 24, 2000

Appl. No.:

09/556390

Inventors:

Kathleen H. Young - Newtown PA
Jian Cao - Monmouth Junction NJ

Assignee:

Wyeth - Madison NJ

International Classification:

G01N 3353

US Classification:

435 71, 82542, 825421

Abstract:

This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a luciferase gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.