Jonathan A Hart

US Patent:

7786298, Aug 31, 2010

Filed:

Jan 12, 2007

Appl. No.:

11/653068

Inventors:

Jacqueline K. Barton - San Marino CA, US
Brian Zeglis - Pasadena CA, US
Irvin H. Lau - Pasadena CA, US
Jonathan Hart - San Diego CA, US
Mi Hee Lim - San Diego CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C07F 1/00
C12Q 1/68
G01N 33/53
A01N 43/04

US Classification:

544225, 435 6, 435 71, 514 44

Abstract:

In accordance with the present invention there are provided sterically demanding intercalators. These compounds are useful for detection of a base-pair mismatch, such as by measuring fluorescence of complexes formed by the compounds of the invention and nucleic acid duplexes. The compounds are also capable of catalyzing photolytic cleavage of nucleic acids.

US Patent:

20060014181, Jan 19, 2006

Filed:

Jun 7, 2005

Appl. No.:

11/147805

Inventors:

Jaqueline Barton - San Marino CA, US
Jonathan Hart - Pasadena CA, US

Assignee:

CALIFORNIA INSTITUTE OF TECHNOLOGY - Pasadena CA

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

The present invention describes methods for directly labeling the 3′-phosphate end at a nucleotide site. Further, as internal 3′-phosphate termini on DNA duplexes are also associated generally with oxidative lesions, these methods provide a general strategy for labeling, and therefore, detecting the frequency of oxidative DNA lesions. The present invention also discloses labeling methods using terminal transferase or nontemplated DNA polymerization, where the use of either of these activities affords tagging a site, after removal of the 3′-phosphate, with polynucleotide tails. Such polynucleotide tails in turn can function as primer binding sites for use in PCR in gene analyses.

US Patent:

20110251973, Oct 13, 2011

Filed:

Apr 8, 2010

Appl. No.:

12/756994

Inventors:

Yanqing Chen - Bellevue WA, US
Jason S. Wodicka - Seattle WA, US
Jonathan R. Hart - Kirkland WA, US

Assignee:

MICROSOFT CORPORATION - Redmond WA

International Classification:

G06Q 99/00

US Classification:

705347

Abstract:

Reviews of products may be analyzed, and statements about the products may be made based on the analysis. Non-professional reviews (e.g., reviews of products written by ordinary consumers of those products) are often difficult to interpret, because different reviewers may apply different standards. When a large number of reviews are available, the reviews can be analyzed statistically to make comparative statements about the products or services reviewed. Sentiments expressed in the reviews may be assigned numerical values. These numerical values for specific products, or classes of products, may be analyzed statistically to determine how the sentiments about a specific product compare with the sentiments about a larger class of products. Using this analysis, a statement can be made, such as, “This television has very good picture quality compared with other televisions of the same price.”