Jonathan J Mcdaniel

US Patent:

20200216840, Jul 9, 2020

Filed:

Jul 27, 2018

Appl. No.:

16/633981

Inventors:

- Austin TX, US
George GEORGIOU - Austin TX, US
Jonathan MCDANIEL - Austin TX, US
Gregory IPPOLITO - Austin TX, US
Andrew ELLINGTON - Austin TX, US

International Classification:

C12N 15/10
G01N 1/28
C12Q 1/6874
C12N 9/12

Abstract:

The present disclosure generally relates to sequencing two or more genes expressed in a single cell in a high-throughput manner using reverse transcriptases. More particularly, the present disclosure relates to a method for high-throughput sequencing of pairs of transcripts co-expressed in single cells (e.g., antibody VH and VL coding sequence) to determine pairs of polypeptide chains that comprise immune receptors.