Kyle Jonathan Schleifer

US Patent:

6870166, Mar 22, 2005

Filed:

Feb 28, 2002

Appl. No.:

10/087619

Inventors:

Bo U. Curry - Redwood City CA, US
Andreas N. Dorsel - Menlo Park CA, US
Kyle J. Schleifer - Los Gatos CA, US
Debra A. Sillman - Los Altos CA, US

Assignee:

Agilent Technologies, Inc. - Palo Alto CA

International Classification:

G01J001/20

US Classification:

2504591, 2504581

Abstract:

A maximum sensitivity optical scanning system is disclosed. It finds use in a variety of applications, including the reading of biopolymeric arrays. It operates by scanning sample at a setting selected to result in signal saturation for some, but not all available data. Subsequent scans of the same area are taken at lower sensitivity settings (in terms of detector gain and/or excitation light source gain or attenuation) and data from at least the previously saturated regions is obtained. If system sensitivity is set too low to produce useful results, optional features may adjust sensitivity upward and follow with an increased sensitivity scan as a remedial measure. Full signal sensitivity is better preserved as most needed in taking data for the weakest signals first with the high-level scan. Data for sample producing stronger signals that can better tolerate photobleaching is then taken in one way or another.

US Patent:

6998230, Feb 14, 2006

Filed:

Apr 26, 2000

Appl. No.:

09/558532

Inventors:

Christopher A. Schantz - Redwood City CA, US
Kyle J. Schleifer - Sunnyvale CA, US
William D. Fisher - San Jose CA, US
Richard P. Tella - Sunnyvale CA, US
Michael P. Caren - Palo Alto CA, US
Peter G. Webb - Menlo Park CA, US

Assignee:

Agilent Technologies, Inc. - Palo Alto CA

International Classification:

C12Q 1/68
C12M 1/36
G01N 15/06

US Classification:

435 6, 4352831, 4352872, 422 58, 422 681, 422100, 347 6, 347 19, 347163, 346 11

Abstract:

A drop deposition apparatus, and a method and computer program products using the drop deposition apparatus, for fabricating at least one addressable array of biopolymers on a substrate. The drop deposition apparatus has a drop dispenser unit and a sensing element. The method comprises includes for each of multiple addresses, dispensing droplets carrying the biopolymers or biopolymer precursors from a drop dispenser unit onto the sensing element, and onto the substrate so as to fabricate the array. Electrical signals resulting from dispensed droplets striking the sensing element are detected. A performance characteristic of the deposition apparatus is evaluated based on the detected signals.

US Patent:

7057185, Jun 6, 2006

Filed:

Aug 2, 2004

Appl. No.:

10/910552

Inventors:

Bo U. Curry - Redwood City CA, US
Andreas N. Dorsel - Menlo Park CA, US
Kyle J. Schleifer - Los Gatos CA, US
Debra A. Sillman - Los Altos CA, US

Assignee:

Agilent Technologies, Inc. - Palo Alto CA

International Classification:

G01J 1/20

US Classification:

2504591, 2504581, 2504612

Abstract:

A maximum sensitivity optical scanning system is disclosed. It finds use in a variety of applications, including the reading of biopolymeric arrays. It operates by scanning sample at a setting selected to result in signal saturation for some, but not all available data. Subsequent scans of the same area are taken at lower sensitivity settings (in terms of detector gain and/or excitation light source gain or attenuation) and data from at least the previously saturated regions is obtained. If system sensitivity is set too low to produce useful results, optional features may adjust sensitivity upward and follow with an increased sensitivity scan as a remedial measure. Full signal sensitivity is better preserved as most needed in taking data for the weakest signals first with the high-level scan. Data for sample producing stronger signals that can better tolerate photobleaching is then taken in one way or another.

US Patent:

7276336, Oct 2, 2007

Filed:

Jul 22, 1999

Appl. No.:

09/359527

Inventors:

Peter G. Webb - Menlo Park CA, US
Michael P. Caren - Palo Alto CA, US
Kyle J. Schleifer - Cupertino CA, US
Jay K. Bass - Mountain View CA, US

Assignee:

Agilent Technologies, Inc. - Palo Alto CA

International Classification:

C12Q 1/68
C12M 1/36
G01N 15/06
B01L 3/02

US Classification:

435 6, 4352831, 4352872, 4352887, 422 681, 422100

Abstract:

A method of fabricating an addressable array of biopolymer probes on a substrate according to a target array pattern using a deposition apparatus, and a deposition apparatus which can execute the method and computer program products for the apparatus. The deposition apparatus which, when operated according to a target drive pattern based on nominal operating parameters of the apparatus, provides the probes on the substrate in the target array pattern. The method includes examining at least one operating parameter for an error from a nominal value which error will result in use of the target drive pattern producing a discrepancy between the target array pattern and an actual array pattern deposited. When an error is detected deriving, based on the error, a corrected drive pattern different from the target drive pattern such that use of the corrected drive pattern results in a reduced discrepancy between the target and actual array patterns.

US Patent:

7531303, May 12, 2009

Filed:

Dec 21, 2001

Appl. No.:

10/036999

Inventors:

Andreas N. Dorsel - Menlo Park CA, US
Kyle J. Schleifer - Sunnyvale CA, US
Elecia C. White - San Jose CA, US
Charles S. Ladd - Union City CA, US
Debra A. Sillman - Santa Clara CA, US

Assignee:

Agilent Technologies, Inc. - Santa Clara CA

International Classification:

C12Q 1/68
C12M 1/00
C12M 1/36
G01N 15/06

US Classification:

435 6, 4352831, 4352872, 4352887, 422 681, 422 8205

Abstract:

A method, apparatus for executing the method, and computer program products for use in such an apparatus. The method includes scanning an interrogating light across multiple sites on an array package including an addressable array of multiple features of different moieties, which scanned sites include multiple array features. Signals from respective scanned sites emitted in response to the interrogating light are detected. The interrogating light power is altered for a first site on the array package during the array scan, based on location of the first site or on a determination that the emitted signal from the first site will be outside a predetermined value absent the altering (which allows for protecting a detector against expected overly bright sites), or is altered during the array scan based on the detected interrogating light power (which allows for compensating for light source drift during an array scan).

US Patent:

20040021055, Feb 5, 2004

Filed:

Jul 31, 2002

Appl. No.:

10/210783

Inventors:

John Corson - Mountain View CA, US
Debra Sillman - Los Altos CA, US
Kyle Schleifer - Los Gatos CA, US
Harry Bunting - San Jose CA, US
Jeffrey McMillan - Morgan Hill CA, US

International Classification:

G01J001/32

US Classification:

250/205000

Abstract:

A biopolymer array optical scanner system that is configured to accommodate the needs of its working environment, but offer extended life over common scanners as typically used, is provided. The scanner is programmed to allow a user to set times or adopt a schedule by which the scanner will automatically power up and/or power down. The activity of the scanner can be controlled by setting a timer or selecting a given time/event, a custom schedule and/or a preselected schedule to trigger action by a software switch at the appointed time. The switch automatically takes such action as previously directed. The activity may be selected from powering up (turning on or going to standby), powering down (turning off or going to standby) and/or initiating a scan run. Myriad combinations or permutations of activities and their respective timing are possible.

US Patent:

20040082059, Apr 29, 2004

Filed:

Oct 21, 2003

Appl. No.:

10/691038

Inventors:

Peter Webb - Menlo Park CA, US
Michael Caren - Palo Alto CA, US
Kyle Schleifer - Cupertino CA, US
Jay Bass - Mountain View CA, US

International Classification:

B05D003/00
C12M001/34

US Classification:

435/287200, 427/002110

Abstract:

A method of fabricating an addressable array of biopolymer probes on a substrate according to a target array pattern using a deposition apparatus, and a deposition apparatus which can execute the method and computer program products for the apparatus. The deposition apparatus which, when operated according to a target drive pattern based on nominal operating parameters of the apparatus, provides the probes on the substrate in the target array pattern. The method includes examining at least one operating parameter for an error from a nominal value which error will result in use of the target drive pattern producing a discrepancy between the target array pattern and an actual array pattern deposited. When an error is detected deriving, based on the error, a corrected drive pattern different from the target drive pattern such that use of the corrected drive pattern results in a reduced discrepancy between the target and actual array patterns.

US Patent:

20060081762, Apr 20, 2006

Filed:

Nov 28, 2005

Appl. No.:

11/288750

Inventors:

John Corson - Mountain View CA, US
Debra Sillman - Los Altos CA, US
Harry Bunting - San Jose CA, US
Kyle Schleifer - Los Gatos CA, US
Jeffrey McMillan - Morgan Hill CA, US

International Classification:

G06F 19/00
G06K 9/00

US Classification:

250205000, 702019000, 382128000

Abstract:

A biopolymer array optical scanner system that is configured to accommodate the needs of its working environment, but offer extended life over common scanners as typically used, is provided. The scanner is programmed to allow a user to set times or adopt a schedule by which the scanner will automatically power up and/or power down. The activity of the scanner can be controlled by setting a timer or selecting a given time/event, a custom schedule and/or a preselected schedule to trigger action by a software switch at the appointed time. The switch automatically takes such action as previously directed. The activity may be selected from powering up (turning on or going to standby), powering down (turning off or going to standby) and/or initiating a scan run. Myriad combinations or permutations of activities and their respective timing are possible.