Lorraine M Wagner

US Patent:

57311633, Mar 24, 1998

Filed:

Nov 22, 1996

Appl. No.:

8/755776

Inventors:

Tina Kangas Vandyk - Wilmington DE
Lorraine Winona Wagner - Newark DE

Assignee:

E. I. du Pont de Nemours and Company - Wilmington DE

International Classification:

C12N 121
C12N 1500
C12Q 166
C12Q 168

US Classification:

435 732

Abstract:

A reagent, useful in the detection of environmental insults comprising bacterial cells containing a stress promoter operably linked to a lux gene complex has been prepared by lyophilizing the cells in a specified medium. The reagent may be used immediately upon rehydration where a positive test for the presence of an environmental insult is given by an increase in light production from the cells.

US Patent:

55996892, Feb 4, 1997

Filed:

May 12, 1995

Appl. No.:

8/440379

Inventors:

Sharon L. Haynie - Philadelphia PA
Lorraine W. Wagner - Newark DE

Assignee:

E. I. du Pont de Nemours and Company - Wilmington DE

International Classification:

C12P 3900

US Classification:

435 42

Abstract:

The present invention provides a process for the biotransformation of a carbohydrate carbon source to 1,3-propanediol using mixed yeast and bacterial cultures wherein the carbohydrate is first fermented to glycerol by the yeast cell and then converted to 1,3-propanediol by the bacterial cell containing an active diol or glycerol dehydratase enzyme in this process both the yeast and bacterial cultures are supported on the same carbon source, and 1,3-propanediol is isolated from the media.