Michael R Buck

US Patent:

6569639, May 27, 2003

Filed:

Jan 18, 2001

Appl. No.:

09/765937

Inventors:

Lance A. Liotta - Potomac MD
Michael E. Buck - Bethesda MD
Rhonda Ann Weiss - Washington DC
Zhengping Zhuang - Bethesda MD
Robert F. Bonner - Washington DC

Assignee:

The United States of America as represented by the Department of Health and Human Services - Washington DC

International Classification:

G01N 130

US Classification:

435 405, 100 4, 422 681

Abstract:

A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.

US Patent:

62040308, Mar 20, 2001

Filed:

Sep 2, 1999

Appl. No.:

9/388805

Inventors:

Lance A. Liotta - Potomac MD
Michael E. Buck - Bethesda MD
Rhonda Ann Weiss - Washington DC
Zhengping Zhuang - Bethesda MD
Robert F. Bonner - Washington DC

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12P 1912

US Classification:

435100

Abstract:

A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.

US Patent:

58436570, Dec 1, 1998

Filed:

Oct 10, 1995

Appl. No.:

8/544388

Inventors:

Lance A. Liotta - Potomac MD
Michael E. Buck - Bethesda MD
Rhonda Weiss - Washington DC
Zhongping Zhuang - Bethesda MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

A01N 102
A01N 100
A01N 300

US Classification:

435 6

Abstract:

A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.

US Patent:

58436448, Dec 1, 1998

Filed:

Mar 1, 1994

Appl. No.:

8/203780

Inventors:

Lance A. Liotta - Potomac MD
Zhengping Zhuang - Rockville MD
Michael R. Buck - Bethesda MD
William G. Stetler-Stevenson - Silver Spring MD
Irina A. Lubensky - Silver Spring MD
Mark J. Roth - Silver Spring MD

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A method of direct extraction of cellular material from a tissue sample which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extracted zone(s) of cells is subjected to analysis. The overall process of identifying, extracting, transporting, and analyzing the extracted zones(s) of cells can be fully automated.

US Patent:

60108886, Jan 4, 2000

Filed:

Sep 8, 1997

Appl. No.:

8/925894

Inventors:

Lance A. Liotta - Potomac MD
Michael E. Buck - Bethesda MD
Rhonda Ann Weiss - Washington DC
Zhengping Zhuang - Bethesda MD
Robert F. Bonner - Washington DC

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12P 1912

US Classification:

435100

Abstract:

A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.