Ning D Wu

US Patent:

7399619, Jul 15, 2008

Filed:

May 24, 2005

Appl. No.:

11/137850

Inventors:

Jianming Xie - San Diego CA, US
Lei Wang - San Diego CA, US
Ning Wu - Boston MA, US
Peter G. Schultz - La Jolla CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12P 21/06
C07H 21/04
C12N 9/00
C12N 1/21
G06F 19/00
G01N 33/48
G01N 33/50

US Classification:

435183, 435193, 435 691, 43525233, 435488, 530350, 536 231, 536 23, 536 241

Abstract:

Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e. g. , brominated or iodinated amino acids. Methods of determining the structure of a protein, e. g. , a protein into which a heavy atom has been site-specifically incorporated through use of an orthogonal tRNA/aminoacyl tRNA-synthetase pair, are also described.

US Patent:

20080233611, Sep 25, 2008

Filed:

Oct 27, 2005

Appl. No.:

11/665083

Inventors:

Peter G. Schultz - La Jolla CA, US
Lital Alfonta - San Diego CA, US
Jonathan R. Chittuluru - San Diego CA, US
Alexander Deiters - Raleigh NC, US
Dan Groff - San Diego CA, US
Daniel Summerer - San Diego CA, US
Meng-Lin Tsao - San Diego CA, US
Jiangyun Wang - La Jolla CA, US
Ning Wu - Boston MA, US
Jianming Xie - San Diego CA, US
Huaqiang Zeng - San Diego CA, US

Assignee:

THE SCRIPPS RESEARCH INSTITUTE - La Jolla CA

International Classification:

C12P 21/06
C12N 9/00
C12P 21/04
C07H 21/04
C12N 15/00
C12N 5/00

US Classification:

435 681, 435196, 435 691, 536 231, 4353201, 435325

Abstract:

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as , or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

US Patent:

20090181858, Jul 16, 2009

Filed:

Sep 21, 2005

Appl. No.:

11/662749

Inventors:

Alexander Deiters - Raleigh NC, US
Ning Wu - Boston MA, US
Peter G. Schultz - La Jolla CA, US
David King - San Francisco CA, US
T. Ashton Cropp - Bethesda MD, US
Mohua Bose - Cupertino CA, US
Dan Groff - San Diego CA, US
Jianming Xie - Mountain View CA, US
Eric Brustad - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C40B 30/06
C12N 9/00
C07K 2/00
C07H 21/00
C12Q 1/68
C12P 21/00
C40B 40/06
C40B 40/02
C40B 30/00

US Classification:

506 10, 435183, 530300, 536 232, 435 6, 435 691, 506 16, 506 14, 506 7

Abstract:

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with a photoregulated amino acid, Ome-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine using these orthogonal pairs.

US Patent:

20130244245, Sep 19, 2013

Filed:

Jun 8, 2012

Appl. No.:

13/492296

Inventors:

Peter G. Schultz - La Jolla CA, US
Lital Alfonta - Omer, IL
Johnathan R. Chittuluru - San Diego CA, US
Alexander Deiters - Saleigh NC, US
Dan Groff - San Diego CA, US
Daniel Summerer - Mannheim, DE
Meng-Lin Tsao - Merced CA, US
Jiangyun Wang - Beijing, CN
Ning Wu - Boston MA, US
Jianming Xie - Mountain View CA, US
Huaqiang Zeng - San Diego CA, US
Mohammad Seyedsayamdost - Newton MA, US
James Turner - San Diego CA, US

Assignee:

The Scripps Research Institute - La Jolla CA

International Classification:

C12N 9/00
C12N 15/10
C12P 21/00

US Classification:

435 618, 435183, 4352542, 43525233, 435 681, 506 1, 435 691, 536 232

Abstract:

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as , or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

US Patent:

20210357333, Nov 18, 2021

Filed:

Jul 30, 2021

Appl. No.:

17/389853

Inventors:

- Shenzhen, CN
Lili Chen - Hopkinton MA, US
Hongliang Tang - Hopkinton MA, US
Ning Wu - Northborough MA, US

International Classification:

G06F 12/0893
G06F 13/28
G06F 15/173

Abstract:

A clustered storage system includes a plurality of storage devices, each of which contributes a portion of its memory to form a global cache of the clustered storage system that is accessible by the plurality of storage devices. Cache metadata for accessing the global cache may be organized in a multi-layered structure. In one embodiment, multi-layered structure has a first layer first including a first address array, and the first address array include addresses pointing to a plurality of second address arrays in a second layer. Each second address array in the second layer includes addresses, each of which points to data that has been cached in the global cache.

US Patent:

20210314400, Oct 7, 2021

Filed:

Jun 15, 2021

Appl. No.:

17/348427

Inventors:

- Shenzhen, CN
Serge Kornfeld - Waltham MA, US
Ning Wu - Northborough MA, US
Xiali He - Upton MA, US

International Classification:

H04L 29/08
H04L 29/06

Abstract:

An embodiment method creates, at a first data center, a first storage volume for an application that is started at the first data center, communicates with a second data center for creating a second storage volume for the application at the second data center to replicate the first storage volume, and sends information identifying the application to the second data center. The second data center creates the second storage volume, and establishes a mapping between the application and the second storage volume using the information identifying the application, where the second data center identifies the second storage volume for the application using the mapping when the application is migrated from the first data center to the second data center.