Peter B Fitzgerald

Address:

3934 Baltimore St, Kensington, MD 20895

VIN:

4T1BK36B37U205419

Make:

TOYOTA

Model:

AVALON

Year:

2007

US Patent:

20200056237, Feb 20, 2020

Filed:

Mar 28, 2018

Appl. No.:

16/495508

Inventors:

- Bethesda MD, US
Peter Fitzgerald - Silver Spring MD, US
Zhili Zheng - Gaithersburg MD, US
Steven A. Rosenberg - Potomac MD, US

Assignee:

The United States of America,as represented by the Secretary,Department of Health and Human Services - Bethesda MD

International Classification:

C12Q 1/6881
C12N 5/0783
C12N 15/10
G16B 30/10
G16B 30/20
C07K 14/725
A61K 35/17

Abstract:

Disclosed are methods of isolating paired T cell receptor (TCR) alpha and beta chain sequences, or an antigen-binding portion thereof. Also disclosed are methods of automatically identifying the TCR alpha and beta chain V segment sequences and CDR3 sequences of a TCR having antigenic specificity for a mutated amino acid sequence encoded by a cancer-specific mutation. Methods of preparing a population of cells that express paired TCR alpha and beta chain sequences, or an antigen-binding portion thereof, are also disclosed. Isolated pairs of TCR alpha and beta chain sequences and isolated populations of cells prepared by the methods are also disclosed.