Robert Keith Burgess

US Patent:

20030135872, Jul 17, 2003

Filed:

Dec 3, 2002

Appl. No.:

10/309757

Inventors:

Robert Burgess - Temecula CA, US
Henry Ji - San Diego CA, US

International Classification:

A01K067/00
A01H001/00
C12N015/82
C12N005/06
C12N005/04
C12N015/85

US Classification:

800/008000, 800/278000, 435/455000, 435/468000, 435/325000, 435/419000

Abstract:

Procedures and vectors are provided for the specific alteration of particular genetic loci in eukaryotic cells. One procedure utilizes fluorescence-probe-in-cell (FPIC) gene targeting DNA vectors for the purpose of creating and identifying cells which have vector sequences integrated into the host cell genome via site-specific homologous recombination. The procedure also utilizes sequences encoding in vivo detectable markers for the identification of cells which have exogenous vector sequences integrated into the genome of the host cell, either via site-specific homologous recombination or nonhomologous recombination or insertion. In addition, cells modified using a FPIC vector, and organisms generated from such cells, are provided.

US Patent:

20040234965, Nov 25, 2004

Filed:

May 23, 2003

Appl. No.:

10/444737

Inventors:

Robert Burgess - Temecula CA, US

International Classification:

A61K048/00
C12Q001/68
C07H021/02

US Classification:

435/006000, 514/044000, 536/023100

Abstract:

The molecular mechanisms by which central nervous system-specific genes are expressed only in the nervous system and repressed in other tissues remain a central issue in developmental and regulatory biology. The presently described invention outlines a dual mechanism of transcriptional downregulation in which the transcription factor REST/NRSF is a central component. The invention delineates short-term REST/NRSF-dependent active repression of neural-specific genes through the recruitment of histone deacetylases as well as long-term gene silencing through the recruitment of a novel biochemical complex which requires CpG methylation. Long-term silencing of neuronal-specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST/NRSF response elements.

US Patent:

20060292560, Dec 28, 2006

Filed:

Aug 8, 2001

Appl. No.:

10/275846

Inventors:

Robert Burgess - Temecula CA, US

International Classification:

C12Q 1/68
G06F 19/00
C12P 19/34
C12N 9/22
C07K 14/705

US Classification:

435006000, 530352000, 702020000, 435091200, 435199000

Abstract:

The ability to rapidly define transcription factor target genes allows for the study of genetic cascades involved in development, physiology and disease. The presently descibed invention outlines the combination of novel chromosomal immunoprecipitation and molecular biology technologies fot the high-throughput in vico discovery and characterization of both known and unknown transcription factor target genes. Through an application of solid phase support matrices and sequential chromosomal immunoprecipitation in combination with molecular cloning procedures this methodology allows for the rapid, stringent purification from cells and tissues of nucleotide sequences representing targets for regulation by transcription factors. Implementation of the technology described herein will result in the efficient simultaneous characterization of both regulatory element and coding sequence target gene information and will be valuable for assessing genetic hierarchies and developing therapeutics.