Skills:
Proficient use of computer applications on both Mac and PC systems: Spreadsheet, word processing, graphical, statistical, data visualization, and other software Quantitative protein analysis: Total protein, gel electrophoresis, western blots, and ELISA assays, including tissue collection, sample preparation, and assay development Immunohistochemistry and in situ hybridization: Tissue fixation, microtome and cryostat sectioning, fluorescent labelling, autoradiography, densitometry, cloning, bacterial transformations, plasmid mini- preps PCR: DNA extraction, isolation, amplification, and gel electrophoresis Handling of small laboratory animals: Colony maintenance (breeding, weaning, genotyping), behavioral analysis (spatial learning, memory, and anxiety), injections, blood draws, surgical procedures (osmotic pump implantation, stereotaxic procedures), post-operative monitoring, euthenasia, tissue collection and micro-dissection Establishment and maintenance of mammalian stem cell cultures under sterile conditions Microscopy: General light, fluorescence and some electron microscopy Handling chemical, biohazardous and radioactive materials Proficient use of computer applications on both Mac and PC systems: Spreadsheet, word processing, graphical, statistical, data visualization, and other software Quantitative protein analysis: Total protein, gel electrophoresis, western blots, and ELISA assays, including tissue collection, sample preparation, and assay development Immunohistochemistry and in situ hybridization: Tissue fixation, microtome and cryostat sectioning, fluorescent labelling, autoradiography, densitometry, cloning, bacterial transformations, plasmid mini- preps PCR: DNA extraction, isolation, amplification, and gel electrophoresis Handling of small laboratory animals: Colony maintenance (breeding, weaning, genotyping), behavioral analysis (spatial learning, memory, and anxiety), injections, blood draws, surgical procedures (osmotic pump implantation, stereotaxic procedures), post-operative monitoring, euthenasia, tissue collection and micro-dissection Establishment and maintenance of mammalian stem cell cultures under sterile conditions Microscopy: General light, fluorescence and some electron microscopy Handling chemical, biohazardous and radioactive materials