Robert J Hickman

US Patent:

8491904, Jul 23, 2013

Filed:

Oct 20, 2010

Appl. No.:

12/908502

Inventors:

Robert K. Hickman - Worcester MA, US

Assignee:

Abbvie Inc. - North Chicago IL

International Classification:

A61K 39/395
A61K 45/00
C07K 16/24
G01N 33/53
G01N 33/537
G01N 33/538

US Classification:

4241451, 424 852, 53038823, 436541, 436824, 435 71

Abstract:

Disclosed herein are methods for the isolation and purification of anti-IL-13 antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography (e. g. , Protein A affinity chromatography), ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.

US Patent:

20100111853, May 6, 2010

Filed:

Oct 20, 2009

Appl. No.:

12/582434

Inventors:

Robert K. Hickman - Worcester MA, US
Qing Huang - Worcester MA, US
Barbara Perilli-Palmer - Bolton MA, US

Assignee:

Abbott Laboratories, Inc. - Abbott Park IL

International Classification:

A61K 51/10
C07K 1/18
C07K 1/34
A61K 39/395
A61K 38/20
A61P 37/00
A61P 19/02
A61P 29/00
A61P 1/00
A61P 3/10
A61P 11/00
A61P 15/00
A61P 25/00
A61P 9/00

US Classification:

424 149, 530416, 530414, 4241301, 4241581, 424 852

Abstract:

Anti-IL-12 antibodies are disclosed herein, including antigen-binding portions thereof. One or more methods for isolating and purifying anti-IL-12 antibodies from a sample matrix is presented. These isolated anti-IL-12 antibodies can be used in a clinical setting as well as in research and development. Pharmaceutical compositions comprising isolated anti-IL-12 antibodies are also described.

US Patent:

20100135987, Jun 3, 2010

Filed:

Oct 20, 2009

Appl. No.:

12/582506

Inventors:

Robert K. Hickman - Worcester MA, US
Qing Huang - Worcester MA, US
Cheryl L. Weed - Scituate RI, US
Scott T. Ennis - Worcester MA, US
Barbara Perilli-Palmer - Bolton MA, US
Min Wan - Frederick MD, US

International Classification:

A61K 39/395
C07K 1/22
A61P 43/00

US Classification:

4241301, 530413

Abstract:

Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.

US Patent:

20100136025, Jun 3, 2010

Filed:

Oct 20, 2009

Appl. No.:

12/582556

Inventors:

Robert K. Hickman - Worcester MA, US
Ivan R. Correia - Winchester MA, US

International Classification:

A61K 39/395
C07K 1/18

US Classification:

4241581, 5303871, 5303892, 5303873, 4241301

Abstract:

Described herein are methods for isolating and purifying antibodies from a sample matrix. One aspect of the present disclosure is directed to viral reduction/inactivation of samples generated in the various steps of antibody purification. In a particular aspect, methods herein employ an acidification step followed by one or more chromatography steps. The chromatography steps can include one or more of the following chromatographic procedures: ion exchange chromatography, affinity chromatography, and hydrophobic interaction chromatography.

US Patent:

20100150864, Jun 17, 2010

Filed:

Oct 20, 2009

Appl. No.:

12/582469

Inventors:

Robert K. Hickman - Worcester MA, US
Qing Huang - Worcester MA, US
Johanna Gervais - West Townsed MA, US

Assignee:

Abbott Laboratories, Inc. - Abbott Park IL

International Classification:

C07K 16/24
A61K 39/395
A61K 38/20

US Classification:

424 852, 5303871, 5303873, 4241581, 5303913

Abstract:

Anti-IL-18 antibodies are disclosed herein, including antigen-binding portions thereof. One or more methods for isolating and purifying anti-IL-18 antibodies from a sample matrix is presented. These isolated anti-IL-18 antibodies can be used in a clinical setting as well as in research and development. Pharmaceutical compositions comprising isolated anti-IL-18 antibodies are also described.

US Patent:

20110301342, Dec 8, 2011

Filed:

Apr 13, 2011

Appl. No.:

13/085630

Inventors:

Chen Wang - Shrewsbury MA, US
Robert K. Hickman - Worcester MA, US
Edwin O. Lundell - Marlborough MA, US
Roy D. Hegedus - Worcester MA, US

Assignee:

ABBOTT LABORATORIES, INC. - Abbott Park IL

International Classification:

C07K 1/36
B01D 15/10

US Classification:

530413, 530417, 530416, 530414, 2101982

Abstract:

The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column.

US Patent:

20120264920, Oct 18, 2012

Filed:

Oct 11, 2011

Appl. No.:

13/270443

Inventors:

Chen Wang - Shrewsbury MA, US
Robert K. Hickman - Worcester MA, US

Assignee:

ABBOTT LABORATORIES - Abbott Park IL

International Classification:

C07K 1/36

US Classification:

5303881, 530417, 530413, 530416, 5303871, 530414

Abstract:

The invention is directed to a method for purifying a protein. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, inactivating viruses in the sample, and processing through at least one depth filter and ion-exchange membrane.

US Patent:

20130195888, Aug 1, 2013

Filed:

Mar 12, 2013

Appl. No.:

13/794881

Inventors:

Chen WANG - Shrewsbury MA, US
Germano COPPOLA - Shrewsbury MA, US
Johanna GERVAIS - West Townsend MA, US
Robert K. HICKMAN - Worcester MA, US
Roy D. HEGEDUS - Worcester MA, US
Gregory J. BUNK - Holden MA, US

Assignee:

AbbVie - North Chicago IL

International Classification:

C07K 1/34

US Classification:

4241581, 530414, 5303892, 5303891, 4241301

Abstract:

Disclosed herein are methods of purifying proteins using ultrafiltration and diafiltration processes.