Scott A Daniels

US Patent:

8569071, Oct 29, 2013

Filed:

Sep 8, 2009

Appl. No.:

12/555319

Inventors:

Subodh B. Nimkar - Foster City CA, US
Subhasish Purkayastha - Acton MA, US
Darryl Pappin - Boxborough MA, US
Scott Daniels - Bolton MA, US

Assignee:

DH Technologies Development Pte. Ltd. - Singapore

International Classification:

G01N 24/00

US Classification:

436173, 436 86, 436111, 435 61, 435 18, 435 23, 435 25, 544358, 544392, 544398, 544399

Abstract:

The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.

US Patent:

20060183238, Aug 17, 2006

Filed:

Feb 8, 2006

Appl. No.:

11/350147

Inventors:

Subodh Nimkar - Foster City CA, US
Subhasish Purkayastha - Acton MA, US
Darryl Pappin - Boxborough MA, US
Scott Daniels - Bolton MA, US

Assignee:

Applera Corporation - Framingham MA

International Classification:

G01N 33/00

US Classification:

436111000

Abstract:

The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.