Steven C March

US Patent:

44302630, Feb 7, 1984

Filed:

Jan 21, 1980

Appl. No.:

6/114021

Inventors:

Steven C. March - Libertyville IL
John W. Safford - Wauconda IL
Susan E. Magic - Lake Bluff IL

Assignee:

Abbott Laboratories - North Chicago IL

International Classification:

G01N 3154
C12N 999
C07G 700

US Classification:

260112R

Abstract:

A specific binding assay method employing, as a labeling substance, a reversible trypsin inhibitor for the detection of a hapten. Competition between the hapten to be determined and hapten trysin inhibitor conjugate for antibody to the hapten, in the presence of enzyme, followed by addition of enzyme substrate provides an effective method for hapten analysis. The preferred trypsin inhibitor is a protein having a molecular weight range of 2,000-75,000. The preferred ratio of the hapten to the inhibitor in the conjugate is between 1:1 and 3:1.

US Patent:

58436340, Dec 1, 1998

Filed:

Jun 4, 1996

Appl. No.:

8/657392

Inventors:

Elaine M Brate - Grayslake IL
Catherine A. Brennan - Libertyville IL
Dominique P. Bridon - Morton Grove IL
Keeve D. Jaffe - Trevor WI
Grant A. Krafft - Glenview IL
Wlodzimierz Mandecki - Libertyville IL
Steven C. March - Libertyville IL
John C. Russell - Greenfield WI
Vincent T. Yue - Deerfield IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C12N 916
C12N 1555
C12Q 144
C07K 1900

US Classification:

435 4

Abstract:

This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.

US Patent:

51749599, Dec 29, 1992

Filed:

Apr 15, 1991

Appl. No.:

7/685317

Inventors:

Samar K. Kundu - Libertyville IL
Richard W. George - Libertyville IL
Steven C. March - Libertyville IL
Sangvorn Rutnarak - Long Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3122

US Classification:

422 59

Abstract:

The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

US Patent:

50717693, Dec 10, 1991

Filed:

Dec 17, 1987

Appl. No.:

7/131811

Inventors:

Samar K. Kundu - Libertyville IL
Richard W. George - Libertyville IL
Steven C. March - Libertyville IL
Sangvorn Rutnarak - Long Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3122

US Classification:

436128

Abstract:

Methods and materials detect ketone and aldehyde analytes in fluid samples by reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.