Susan L Welch

US Patent:

51358549, Aug 4, 1992

Filed:

May 3, 1988

Appl. No.:

7/189547

Inventors:

Vivian L. MacKay - Seattle WA
Susan K. Welch - Seattle WA
Carli L. Yip - Bellevue WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

C12N 1500
C12N 1581
C12N 119
C12P 2102

US Classification:

435 691

Abstract:

Methods for producing a heterologous protein or polypeptide are disclosed. A preferred method utilizes a fungal cell carrying a defect in a gene whose product is required for the addition of outer chain oligosaccharide moieties to glycoproteins, the cell transformed with a first DNA construct comprising a regulated promoter followed downstream by a DNA sequence which complements the defect, and a second DNA construct comprising a second promoter followed downstream by a DNA sequence encoding a secretion signal and a DNA sequence encoding a heterologous protein or polypeptide. A yeast cell having a Mnn9. sup. - phenotype and capable of producing colonies of normal morphologies in the absence of osmotic stabilization is also disclosed.