Yun Ja Kang

US Patent:

7875446, Jan 25, 2011

Filed:

Apr 19, 2007

Appl. No.:

11/788071

Inventors:

Yun Kang - Livingston NJ, US
Mark William Cutler - Bloomingdale NJ, US
Amadou Affrey Ouattara - New Milford NJ, US
Kristen Elissa Syvertsen - Tarrytown NY, US

Assignee:

Wyeth LLC - Madison NJ

International Classification:

C12N 7/02
C12N 1/02
C12N 7/01
A61K 39/205
A61K 39/38
A61K 39/12
A01N 63/00
A61K 39/23

US Classification:

435239, 4242241, 4241841, 4242041, 424 936, 424 931, 435261, 4352351

Abstract:

A process is described for purifying vesicular stomatitis virus (VSV) from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0. 2 to 0. 45 μm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0. 2 to 0. 22 μm filter and recovering the VSV in the filtered solution.

US Patent:

20060246544, Nov 2, 2006

Filed:

Mar 30, 2006

Appl. No.:

11/396215

Inventors:

Yun Kang - Livingston NJ, US
Walter Willett - Doylestown PA, US
Thomas Klimek - Philadelphia PA, US
Basil Campbell - Gaithersburg MD, US
Paul Cino - Jamison PA, US
Bradley Thomas - Quakertown PA, US
John Bermel - Telford PA, US
Chun-Chiang Chen - Gaithersburg MD, US

Assignee:

Neose Technologies,Inc. - Horsham PA

International Classification:

C12P 21/06
C12N 9/24
C12N 5/06
C07K 14/53
C07K 14/51

US Classification:

435069100, 435320100, 435348000, 435200000, 530351000, 530414000, 530399000

Abstract:

The present invention provides a manufacturing method for the production of peptides that are grown in insect cell lines. The peptides are grown in insect cell cultures that are infected with baculovirus particles in a culture supplemented with a lipid mixture. The peptides are then isolated from the insect cell culture using a method that employs a tangential flow filtration cascade. The isolated peptides are glycopeptides having an insect specific glycosylation pattern. The glycopeptides may then be conjugated to a modifying group via linkage through a glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase.