Amber N Johnson

US Patent:

20200124613, Apr 23, 2020

Filed:

Dec 10, 2019

Appl. No.:

16/709903

Inventors:

- Austin TX, US
Eric V. ANSLYN - Austin TX, US
Andrew ELLINGTON - Austin TX, US
Jagannath SWAMINATHAN - Austin TX, US
Erik HERNANDEZ - Austin TX, US
Amber JOHNSON - Austin TX, US
Alexander BOULGAKOV - Austin TX, US
James L. BACHMAN - Austin TX, US
Helen SEIFERT - Austin TX, US

International Classification:

G01N 33/68
G01N 33/58
C07K 17/08

Abstract:

Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.

US Patent:

20200018768, Jan 16, 2020

Filed:

Sep 16, 2019

Appl. No.:

16/572194

Inventors:

- Austin TX, US
Eric V. ANSLYN - Austin TX, US
Andrew ELLINGTON - Austin TX, US
Jagannath SWAMINATHAN - Austin TX, US
Erik HERNANDEZ - Austin TX, US
Amber JOHNSON - Austin TX, US
Alexander BOULGAKOV - Austin TX, US
James L. BACHMAN - Austin TX, US
Helen SEIFERT - Austin TX, US

International Classification:

G01N 33/68
G01N 33/58

Abstract:

Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.

US Patent:

20170276686, Sep 28, 2017

Filed:

Sep 15, 2015

Appl. No.:

15/510962

Inventors:

- Austin TX, US
Eric V. Anslyn - Austin TX, US
Andrew Ellington - Austin TX, US
Jagannath Swaminathan - Marharastra, IN
Erik Hernandez - Austin TX, US
Amber Johnson - Austin TX, US
Alexander Boulgakov - Austin TX, US
James L. Bachman - Austin TX, US
Helen Seifert - Austin TX, US

International Classification:

G01N 33/68
G01N 33/58

Abstract:

The present invention relates to the field of identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level. The present invention also relates to methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.