Andrew J Main

US Patent:

20120196315, Aug 2, 2012

Filed:

Feb 1, 2012

Appl. No.:

13/363588

Inventors:

Lishan Zhao - Emeryville CA, US
Lan Xu - San Diego CA, US
Patrick Westfall - Novato CA, US
Andrew Main - Berkeley CA, US

Assignee:

AMYRIS, INC. - Emeryville CA

International Classification:

C12Q 1/02

US Classification:

435 29

Abstract:

The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.

US Patent:

20120270260, Oct 25, 2012

Filed:

Jul 5, 2012

Appl. No.:

13/542491

Inventors:

Lishan Zhao - Emeryville CA, US
Lan Xu - San Diego CA, US
Patrick Westfall - Novato CA, US
Andrew Main - Berkeley CA, US

Assignee:

AMYRIS, INC. - Emeryville CA

International Classification:

C12Q 1/02
C12N 1/21
C12N 1/19

US Classification:

435 29, 4352542, 43525421, 4352523, 43525233

Abstract:

The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.

US Patent:

20220251606, Aug 11, 2022

Filed:

Mar 9, 2022

Appl. No.:

17/690429

Inventors:

- Emeryville CA, US
Andrew MAIN - Benicia CA, US
Chia-Hong TSAI - Martinez CA, US

International Classification:

C12N 15/90
C12N 15/10
C12N 15/81

Abstract:

Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.

US Patent:

20190144887, May 16, 2019

Filed:

May 18, 2017

Appl. No.:

16/302079

Inventors:

- Emeryville CA, US
Andrew MAIN - Benicia CA, US
Chia-Hong TSAI - Martinez CA, US

International Classification:

C12N 15/90
C12N 15/10

Abstract:

Provided herein are compositions and methods of integrating one or more exogenous donor nucleic acids into one or more exogenous landing pads engineered into a host cell's genome. In certain embodiments, the exogenous landing pads and exogenous donor nucleic acids comprise standardized, compatible homology regions so that exogenous donor nucleic acids can integrate into any of the landing pads, independent of the genomic sequences surrounding the landing pads. In certain embodiments, the methods comprise contacting the host cell comprising landing pads with one or more exogenous donor nucleic acids, and a nuclease capable of causing a double-strand break within the landing pads, and recovering a host cell comprising one or more exogenous donor nucleic acids integrated in any of the landing pads.