Bin Yang

US Patent:

7604967, Oct 20, 2009

Filed:

Mar 19, 2003

Appl. No.:

10/391740

Inventors:

Bin Yang - Hanover NH, US
Charles E. Wyman - Norwich VT, US

Assignee:

The Trustees of Dartmouth College - Hanover NH

International Classification:

C12P 7/06

US Classification:

435161, 530378, 435 681, 435 99

Abstract:

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion. Cellulase efficiencies are improved by the protein or polypeptide treatment. The treatment may be used in combination with steam explosion and acid prehydrolysis techniques. Hydrolysis yields from lignin containing biomass are enhanced 5-20%, and enzyme utilization is increased from 10% to 50%. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.

US Patent:

7875444, Jan 25, 2011

Filed:

Sep 19, 2005

Appl. No.:

11/229817

Inventors:

Bin Yang - West Lebanon NH, US
Charles E. Wyman - Norwich VT, US

Assignee:

The Trustees of Dartmouth College - Hanover NH

International Classification:

C12P 7/06
C12P 7/08
C12P 7/10
C12P 19/14
C12P 21/06
C07K 14/415
C07K 14/765

US Classification:

435161, 435 681, 435 99, 435163, 435165, 530363, 530370, 530378

Abstract:

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.

US Patent:

8580541, Nov 12, 2013

Filed:

Dec 7, 2010

Appl. No.:

12/962366

Inventors:

Bin Yang - Richland WA, US
Charles E. Wyman - Riverside CA, US

Assignee:

The Trustees of Dartmouth College - Hanover NH

International Classification:

C12P 7/06
C12P 7/08
C12P 7/10
C12P 19/14
C12P 21/06
C07K 14/415
C07K 14/765

US Classification:

435161, 435 681, 435 99, 435163, 435165, 530363, 530370, 530378

Abstract:

Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein.