Brian J Gagnon

US Patent:

8137561, Mar 20, 2012

Filed:

Jun 23, 2011

Appl. No.:

13/167297

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

210635, 210656, 2101982, 2105021

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

8435406, May 7, 2013

Filed:

Feb 1, 2012

Appl. No.:

13/363397

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

2101982, 2105021, 210635, 210656

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities even at high conductivities and pH.

US Patent:

20090050566, Feb 26, 2009

Filed:

Aug 4, 2008

Appl. No.:

12/221496

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

International Classification:

B01D 15/36
B32B 9/00
B05D 3/02

US Classification:

210656, 4283184, 210506, 4273855, 427551

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

20100200507, Aug 12, 2010

Filed:

Apr 21, 2010

Appl. No.:

12/764340

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

MILLIPORE CORPORATION - Billerica MA

International Classification:

B01D 15/08

US Classification:

210656

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

20110284446, Nov 24, 2011

Filed:

Jun 23, 2011

Appl. No.:

13/167291

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

MILLIPORE CORPORATIOIN - Billerica MA

International Classification:

B01D 15/36
B01J 41/20
B01D 15/08

US Classification:

2101982, 2105021, 21050027, 21050037

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

US Patent:

20120121819, May 17, 2012

Filed:

Dec 29, 2011

Appl. No.:

13/339503

Inventors:

Mikhail Kozlov - Belmont MA, US
Wilson Moya - Concord MA, US
Michael W. Phillips - Tyngsborough MA, US
Senthilkumar Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US

Assignee:

MILLIPORE CORPORATIOIN - Billerica MA

International Classification:

B05D 5/00
B05D 3/06

US Classification:

427551, 427244

Abstract:

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (e.g., Protein A or Protein G) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.