Brian R Gagnon

US Patent:

7678269, Mar 16, 2010

Filed:

Sep 13, 2006

Appl. No.:

11/520848

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Georgetown NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - StoneHame MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

2101982, 2105021, 210635, 210656, 502404

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

US Patent:

7678302, Mar 16, 2010

Filed:

Sep 13, 2006

Appl. No.:

11/520775

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin Umana - StoneHame MA, US
Neil P. Soice - Amherst NH, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B29B 9/00

US Classification:

264 5, 264 7

Abstract:

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads.

US Patent:

8177537, May 15, 2012

Filed:

Mar 2, 2010

Appl. No.:

12/660708

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Umana Joaquin - Stoneham MA, US
Neil Soice - Amherst NH, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

B29B 9/00

US Classification:

425 6, 425 10, 366341

Abstract:

The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads.

US Patent:

20100174051, Jul 8, 2010

Filed:

Mar 2, 2010

Appl. No.:

12/660715

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - Stoneham MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

Millipore Corporation - Billerica MA

International Classification:

C07K 1/22

US Classification:

5303881

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

US Patent:

20120006752, Jan 12, 2012

Filed:

May 6, 2011

Appl. No.:

13/102081

Inventors:

KS Cheng - Nashua NH, US
Senthil Ramaswamy - Singapore, SG
Brian Gagnon - Billerica MA, US
Maybelle Woo - Braintree MA, US
Mikhail Kozlov - Belmont MA, US

Assignee:

MILLIPORE CORPORATION - Billerica MA

International Classification:

B01D 15/08
B01D 39/18

US Classification:

210660, 2105021

Abstract:

Media and devices, such as nitrocellulose-based filters, wherein the media is coated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

US Patent:

20120168381, Jul 5, 2012

Filed:

Mar 8, 2012

Appl. No.:

13/414746

Inventors:

Senthil Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US
Maybelle Woo - Braintree MA, US
KS Cheng - Nashua NH, US
Mikhail Kozlov - Belmont MA, US

Assignee:

EMD MILLIPORE CORPORATION - Billerica MA

International Classification:

B01D 39/18
B01D 15/00

US Classification:

210663, 210496

Abstract:

Media and devices, such as depth filters including such media, wherein the media is impregnated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

US Patent:

20120168382, Jul 5, 2012

Filed:

Mar 8, 2012

Appl. No.:

13/414747

Inventors:

KS Cheng - Nashua NH, US
Senthil Ramaswamy - Nashua NH, US
Brian Gagnon - Billerica MA, US
Maybelle Woo - Braintree MA, US
Mikhail Kozlov - Belmont MA, US

Assignee:

EMD MILLIPORE CORPORATION - Billerica MA

International Classification:

B01D 15/00

US Classification:

210663

Abstract:

Media and devices, such as nitrocellulose-based filters, wherein the media is coated with a polymer such as a polyallylamine. The resulting device offers strong binding of protein impurities and superior removal of host cell proteins from biological samples.

US Patent:

20140073769, Mar 13, 2014

Filed:

Nov 19, 2013

Appl. No.:

14/083590

Inventors:

Senthilkumar Ramaswamy - Nashua NH, US
Chen Wang - Acton MA, US
Nanying Bian - Lexington MA, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - Reading MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Groton MA, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

C07K 16/00

US Classification:

5303881

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.