Chen Wang

US Patent:

7678269, Mar 16, 2010

Filed:

Sep 13, 2006

Appl. No.:

11/520848

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Georgetown NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - StoneHame MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

Millipore Corporation - Billerica MA

International Classification:

B01D 15/08

US Classification:

2101982, 2105021, 210635, 210656, 502404

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

US Patent:

7833723, Nov 16, 2010

Filed:

Jan 5, 2007

Appl. No.:

11/650093

Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

G01N 33/53

US Classification:

435 71, 436518

Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

US Patent:

7846682, Dec 7, 2010

Filed:

Nov 13, 2009

Appl. No.:

12/590726

Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

G01N 33/53

US Classification:

435 71, 436518

Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

US Patent:

8114611, Feb 14, 2012

Filed:

Nov 1, 2010

Appl. No.:

12/916978

Inventors:

Nanying Bian - Nashua NH, US
John Charkoudian - Carlisle MA, US
Neil Soice - Amherst NH, US
Joaquin Umana - Stoneham MA, US
Chen Wang - Acton MA, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

G01N 33/53

US Classification:

435 71, 435 72, 436518

Abstract:

The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

US Patent:

20090246885, Oct 1, 2009

Filed:

Jul 7, 2008

Appl. No.:

12/217558

Inventors:

Nanying Bian - Nashua NH, US
Neil Soice - Amherst NH, US
Chen Wang - Acton MA, US
Yuan Wong - Booton NJ, US

Assignee:

Millipore Corporation - Billerica MA

International Classification:

G01N 33/566
B32B 5/16
G01N 33/552
G01N 33/558

US Classification:

436501, 428402, 436527, 436514

Abstract:

The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

US Patent:

20100174051, Jul 8, 2010

Filed:

Mar 2, 2010

Appl. No.:

12/660715

Inventors:

Kwok-Shun Cheng - Nashua NH, US
Senthilkumar Ramaswamy - Nashua NH, US
Chen Wang - Acton MA, US
Nanying Bian - Nashua NH, US
Brian Gagnon - Billerica MA, US
Joaquin A. Umana - Stoneham MA, US
Dennis Aquino - Chelmsford MA, US
Neil Soice - Amherst NH, US
Lyddiatt Andrew - Co Durham, GB

Assignee:

Millipore Corporation - Billerica MA

International Classification:

C07K 1/22

US Classification:

5303881

Abstract:

The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

US Patent:

20110301342, Dec 8, 2011

Filed:

Apr 13, 2011

Appl. No.:

13/085630

Inventors:

Chen Wang - Shrewsbury MA, US
Robert K. Hickman - Worcester MA, US
Edwin O. Lundell - Marlborough MA, US
Roy D. Hegedus - Worcester MA, US

Assignee:

ABBOTT LABORATORIES, INC. - Abbott Park IL

International Classification:

C07K 1/36
B01D 15/10

US Classification:

530413, 530417, 530416, 530414, 2101982

Abstract:

The invention is directed to an apparatus and method for purifying a protein. The apparatus involves the use of a capture chromatography resin, a depth filter arranged after the capture chromatography resin, and a mixed-mode chromatography resin arranged after the depth filter. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, a depth filter, and a mixed-mode chromatography resin. A membrane adsorber or monolith may be substituted for the mixed-mode chromatography column.

US Patent:

20120264920, Oct 18, 2012

Filed:

Oct 11, 2011

Appl. No.:

13/270443

Inventors:

Chen Wang - Shrewsbury MA, US
Robert K. Hickman - Worcester MA, US

Assignee:

ABBOTT LABORATORIES - Abbott Park IL

International Classification:

C07K 1/36

US Classification:

5303881, 530417, 530413, 530416, 5303871, 530414

Abstract:

The invention is directed to a method for purifying a protein. The method involves providing a sample containing the protein, processing the sample through a capture chromatography resin, inactivating viruses in the sample, and processing through at least one depth filter and ion-exchange membrane.