Daniel F Knowles

US Patent:

60938064, Jul 25, 2000

Filed:

Oct 10, 1996

Appl. No.:

8/728603

Inventors:

Ethel Cesarman - Hoboken NJ
Daniel M. Knowles - Forest Hills NY

Assignee:

Cornell Research Foundation, Inc. - Ithaca NY

International Classification:

C07H 2104

US Classification:

536 231

Abstract:

The present invention is directed to isolated nucleic acid molecules encoding proteins of Kaposi's sarcoma associated herpesvirus, including an antigenic receptor protein, a G protein coupled receptor, and a cyclin protein. Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for increasing or decreasing the expression of the KSHV proteins in host cells. DNA oligomers and antibodies specific for the KSHV proteins are provided, each of which can be used to detect the KSHV proteins in a sample. Isolated KSHV proteins are also provided.

US Patent:

59087730, Jun 1, 1999

Filed:

Sep 20, 1996

Appl. No.:

8/717291

Inventors:

Ethel Cesarman - Hoboken NJ
Leandros Arvanitakis - New York NY
Daniel M. Knowles - Forest Hills NY
Enrique Mesri - Bronx NY

Assignee:

Cornell Research Foundation, Inc. - Ithaca NY

International Classification:

C12N 700
A61K 39245

US Classification:

4352351

Abstract:

The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.