David Jay Garfinkel

US Patent:

60905549, Jul 18, 2000

Filed:

Oct 31, 1997

Appl. No.:

8/963602

Inventors:

Richard Woychik - Beechwood OH
David Garfinkel - Frederick MD

Assignee:

Amgen, Inc. - Thousand Oaks CA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The present invention is directed to methods for producing or obtaining gene targeting constructs by way of homologous recombination in host cells and to targeting constructs produced by those methods. The invention is also directed to transgenic animals having targeted mutations introduced in to the cells of the animal using the targeting constructs of the invention.

US Patent:

57143137, Feb 3, 1998

Filed:

May 24, 1995

Appl. No.:

8/449207

Inventors:

David J. Garfinkel - Frederick MD
Dwight V. Nissley - Frederick MD
Joan M. Curcio - Albany NY
Jeffrey N. Strathern - Frederick MD

Assignee:

United States of America as represented by the Department of Health and
Human Services - Washington DC

International Classification:

C12Q 170
C12N 119
C12N 1581

US Classification:

435 5

Abstract:

The present invention relates, in general, to a DNA segment. In particular, the present invention relates to a DNA segment comprising a selectable marker gene, a DNA segment comprising a selectable marker gene inserted into a retrotransposon, cells containing these DNA segments, and methods of using these DNA segments and cells. The present invention further relates to a vector comprising a selectable marker gene inserted into a retrotransposon, wherein the retrotransposon comprises a retroviral reverse transcriptase/RNase H gene domain and wherein the selectable marker gene contains an intron inserted into a coding sequence of the marker gene and the intron is in antisense orientation relative to transcription of the marker gene and in sense orientation relative to transcription of the retrotransposon.

US Patent:

54628733, Oct 31, 1995

Filed:

Aug 6, 1993

Appl. No.:

8/102854

Inventors:

David J. Garfinkel - Frederick MD
M. Joan Curcio - Keedysville MD

Assignee:

The United States of America as represented by the Department of Health
and Human Services - Washington DC

International Classification:

C12N 1500
C07H 1700

US Classification:

4352542

Abstract:

The present invention relates, in general, to a DNA segment. In particular, the present invention relates to a DNA segment comprising a selectable marker gene, a DNA segment comprising a selectable marker gene inserted into a retrotransposon, cells containing these DNA segments, and methods of using these DNA segments and cells.