Eric J Paquette

US Patent:

20230051903, Feb 16, 2023

Filed:

Jan 15, 2021

Appl. No.:

17/758908

Inventors:

- Wayzata MN, US
Matthew GUTZMANN - Blair NE, US
Christopher Lee NEUMANN - Omaha NE, US
Eric PAQUETTE - Dayton OH, US
Victor SHERONY - Omaha NE, US
Joseph SPENCER - Bennington NE, US

Assignee:

CARGILL, INCORPORATED - Wayzata MN

International Classification:

C12P 7/14
C12P 7/40
C12P 19/00

Abstract:

A fermentation process includes contacting a starch hydrolysate with a glucoamylase with agitation. And allowing for settling to form a multi-phase solution. A first phase of the multi-phase solution includes a saccharide component comprising about 30 wt % to about 80 wt % (e.g., 30 wt % to 70 wt %, 30 wt % to 60 wt %) based on a total carbohydrate present. The method further includes draining the first phase to isolate the first phase from a second phase to form a fermentation broth comprising a first portion of the first phase. The method further includes fermenting the fermentation broth until a concentration of glucose in the fermentation broth is 40 g/L or less. The method includes adding a second portion of the first phase to the fermentation broth to maintain a concentration of glucose in a range of from about 1 g/L to about 20 g/L in the fermentation broth.