wbpL was PCR-amplified from P. aeruginosa PAO1 genomic DNA and inserted into the expression vector pSEVA524 (Rubn de Dios, Eduardo Santero and Francisca Reyes-Ramrez, unpublished)78 by Gibson assembly (NEB Hifi Assembly, NEB Location). After conjugation with E. coli JKE201 and selection on LB 10