Hong Y Tan

US Patent:

6685813, Feb 3, 2004

Filed:

Aug 20, 2001

Appl. No.:

09/933993

Inventors:

Stephen J. Williams - San Mateo CA
Hong Dong Tan - San Jose CA
Hung Pin Kao - Fremont CA
Wyatt N. Vreeland - Chicago IL

Assignee:

Aclara Biosciences, Inc. - Mountain View CA

International Classification:

B01D 5902

US Classification:

204549

Abstract:

A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected, in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charged sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separate by zone electrophoresis.

US Patent:

6818113, Nov 16, 2004

Filed:

Feb 10, 2001

Appl. No.:

09/780638

Inventors:

Stephen J. Williams - San Mateo CA
Hong Dong Tan - San Jose CA
Hung Pin Kao - Fremont CA
Wyatt N. Vreeland - Chicago IL

Assignee:

Aclara Biosciences, Inc. - Mountain View CA

International Classification:

B01D 5702

US Classification:

204453, 204601, 204450

Abstract:

A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.

US Patent:

6845634, Jan 25, 2005

Filed:

Jun 20, 2002

Appl. No.:

10/175945

Inventors:

Hong Tan - Fremont CA, US
Youmin Liu - Palo Alto CA, US
Yushan Tan - Shanghai, CN

Assignee:

Wave Crossing Corporation - Palo Alto CA

International Classification:

C03B 2500

US Classification:

65 3013, 65 3014, 65114

Abstract:

A fabrication method for a thallium-doped GRIN lens composed of a simplified glass composition is described. The composition is composed of silicon, oxygen, boron, zinc, potassium, sodium, and thallium. The method is comprised of preparing a cylindrical preform, annealing the cylindrical preform, removing contaminants from preform thereafter extruded to form a glass rod, exposing the glass rod to an alkali salt bath, and chemically milling the glass rod within an acid bath. Method and composition produce colorless lenses with a refractive index from 1. 5900 to 1. 7810, an insertion loss no greater than 0. 05 dB, and a chromatic transmittance greater than 90 percent.

US Patent:

7319525, Jan 15, 2008

Filed:

Jun 12, 2006

Appl. No.:

11/423671

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Krista Leah Witte - Hayward CA, US
Robert Zuk - Atherton CA, US
Greg L. Carricato - San Jose CA, US
Scott Lockard - Los Gatos CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.

US Patent:

7394547, Jul 1, 2008

Filed:

Nov 4, 2004

Appl. No.:

10/981901

Inventors:

Hong Tan - San Jose CA, US
Duan Jun Chen - East Brunswick NJ, US
Yushan Tan - Shanghai, CN
Krista Leah Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356480

Abstract:

Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.

US Patent:

7494577, Feb 24, 2009

Filed:

Sep 30, 2003

Appl. No.:

10/676857

Inventors:

Stephen J. Williams - San Mateo CA, US
Hong Dong Tan - San Jose CA, US
Hung Pin Kao - Fremont CA, US
Wyatt N. Vreeland - Chicago IL, US

Assignee:

Monogram Biosciences, Inc. - South San Francisco CA

International Classification:

G01N 27/447

US Classification:

204645, 204549, 204601

Abstract:

A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte. With the application of a voltage potential across the microchannel, charged components in the sample stack by isotachophoresis, and electrolytic hydroxyl or hydrogen ions formed by electrolysis at the upstream-end electrode migrate into the trailing-edge ion buffer, titrating the titratable species therein, where the concentration of the titratable species in the trailing-edge electrolyte is selected , in relation to the lengths of the upstream channel region and sample-loading volume, to permit the sample to stack into a relatively small sample volume before electrolytic-ion migration from the upstream electrode into and through the sample-volume region is effective to overtake the charge sample components. With continued application of an electric potential across the channel ends, charged sample components in the stacked sample volume separated by zone electrophoresis.

US Patent:

7656536, Feb 2, 2010

Filed:

Dec 14, 2007

Appl. No.:

11/957340

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Krista Leah Witte - Hayward CA, US
Greg L. Carricato - San Jose CA, US
Scott Lockard - Los Gatos CA, US

Assignee:

Fortebio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.

US Patent:

7728982, Jun 1, 2010

Filed:

Apr 8, 2008

Appl. No.:

12/099751

Inventors:

Hong Tan - San Jose CA, US
Yushan Tan - Shanghai, CN
Duan Jun Chen - East Brunswick NJ, US
Krista Leah Witte - Hayward CA, US

Assignee:

ForteBio, Inc. - Menlo Park CA

International Classification:

G01B 9/02

US Classification:

356478, 356480

Abstract:

Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.