Hua Lin

US Patent:

7087896, Aug 8, 2006

Filed:

Dec 27, 2004

Appl. No.:

11/023234

Inventors:

Christopher H. Becker - Palo Alto CA, US
Curtis A. Hastings - Bethesda MD, US
Scott M. Norton - Durham NC, US
Sushmita Mimi Roy - Santa Clara CA, US
Weixun Wang - Mountain View CA, US
Haihong Zhou - Mountain View CA, US
Thomas Andrew Shaler - Fremont CA, US
Praveen Kumar - Santa Clara CA, US
Markus Anderle - Campbell CA, US
Hua Lin - Sunnyvale CA, US

Assignee:

PPD Biomarker Discovery Sciences, LLC - Wilmington NC

International Classification:

B01D 59/44
H01J 49/00

US Classification:

250282, 250281

Abstract:

Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

US Patent:

20020155601, Oct 24, 2002

Filed:

Jan 2, 2002

Appl. No.:

10/032495

Inventors:

Wen Yan - Potomac MD, US
Steve Huang - Germantown MD, US
Minh-Thanh Nguyen - Rockville MD, US
Hua Lin - Potomac MD, US
Jingqi Lei - Gaithersburg MD, US
Ruchi Khanna - Gemantown MD, US

International Classification:

A61K048/00
C12Q001/68
C12N005/08

US Classification:

435/366000, 435/006000, 424/093210

Abstract:

A method of producing a homogenous population of homozygous stem (HS) cells pre-selected for immunotype and/or genotype from donor cells is described herein. The invention relates to methods of using immunohistocompatible HS cells for diagnosis, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, traumatic injuries and cancer. The invention further relates to methods for using histocompatible HS stem cells pre-selected for a non-disease genotype for prophylactic and therapeutic intervention including, but not limited to, therapeutic and cosmetic transplantation, and the treatment of various genetic diseases, neurodegenerative diseases, and cancer. Furthermore, the invention relates to a catalogued transplant depository of HS cells derived from multiple donors, each of the HS cells being homozygous for a unique HLA haplotype, for the purpose of having a constant, reliable, comprehensive supply of immunohistocompatible cells for diagnosis, treatment and/or transplantation.

US Patent:

20020168763, Nov 14, 2002

Filed:

Nov 30, 2001

Appl. No.:

09/997240

Inventors:

Wen Yan - Potomac MD, US
Steve Huang - Germantown MD, US
Minh-Thanh Nguyen - Rockville MD, US
Hua Lin - Potomac MD, US
Jingqi Lei - Gaithersburg MD, US
Ruchi Khanna - Germantown MD, US

International Classification:

C12N005/06
C12N005/08

US Classification:

435/325000, 435/354000, 435/366000, 435/350000

Abstract:

The present invention discloses and describes pluripotent homozygous stem (HS) cells, and methods and materials for making same. The present invention also provides methods for differentiation of HS cells into progenitor (multipotent) cells or other desired cells, groups of cells or tissues. Further, the applications of the HS cells disclosed herein, include (but are not limited to) the diagnosis and treatment of various diseases (for example, genetic diseases, neurodegenerative diseases, endocrine-related disorders and cancer), traumatic injuries, cosmetic or therapeutic transplantation, gene therapy and cell replacement therapy.

US Patent:

20040053272, Mar 18, 2004

Filed:

Mar 3, 2003

Appl. No.:

10/377033

Inventors:

Steven Huang - Germantown MD, US
Hua Lin - North Potomac MD, US

International Classification:

C12Q001/68
G01N033/53
G01N033/567
C12N005/08

US Classification:

435/006000, 435/007200, 435/455000, 435/366000

Abstract:

The present invention relates to materials and methods for the quantitative and qualitative analysis of gene expression during targeted differentiation of isolated homozygous stem cells, and to materials and methods for accumulating sequence tags sampled from a population of expressed genes. More particularly, the present invention relates to a method for constructing a model of cellular development and differentiation comprising the steps of: (1) producing a homogenous population of homozygous stem (HS) cells; (2) directing the differentiation of the HS cells to arrive at desired mature (somatic) cells, group of cells or tissue; (3) periodically sampling HS cells undergoing differentiation to extract total cellular RNA produced at various sampling stages; (4) isolating mRNA from the total cellular RNA; and (5) constructing a cDNA library representative of proteins produced and expressed at various stages of differentiation, over the course of time. The present invention further relates to sequencing and cataloguing isolated mRNAs and cDNAs at various stages of differentiation of HS cells, producing and cataloguing isolated polypeptides sequences encoded by same, and screening the cDNA libraries. In vitro and in vivo cultures of homozygous stem cell are also described; such cultures find utility both as models of differentiation as well as sources of differentiated tissues and organs.

US Patent:

20080260707, Oct 23, 2008

Filed:

Apr 4, 2008

Appl. No.:

12/078799

Inventors:

Wen Liang Yan - Potomac MD, US
Steve Chien-Wen Huang - Germantown MD, US
Minh-Thanh Nguyen - Rockville MD, US
Hua Helen Lin - North Potomac MD, US
Jingqi Lei - Gaithersburg MD, US
Ruchi Khanna - Germantown MD, US

Assignee:

Stemron, Inc. - Rockville MD

International Classification:

A61K 35/12
A61K 48/00
C12N 5/08
C12N 15/63
A61P 43/00

US Classification:

424 9321, 435455, 435366

Abstract:

The present invention discloses and describes pluripotent homozygous stem (HS) cells, and methods and materials for making same. The present invention also provides methods for differentiation of HS cells into progenitor (multipotent) cells or other desired cells, groups of cells or tissues. Further, the applications of the HS cells disclosed herein, include (but are not limited to) the diagnosis and treatment of various diseases (for example, genetic diseases, neurodegenerative diseases, endocrine-related disorders and cancer), traumatic injuries, cosmetic or therapeutic transplantation, gene therapy and cell replacement therapy.

US Patent:

20100062471, Mar 11, 2010

Filed:

Sep 29, 2006

Appl. No.:

12/088228

Inventors:

Aaron B. Kantor - San Carlos CA, US
Susan Goelz - Portland OR, US
Jun Deng - Cupertino CA, US
Hua Lin - Fremont CA, US
Christopher Becker - Palo Alto CA, US

Assignee:

PPD BIOMARKER DISCOVERY SCIENCES LLC - Wilmington NC
BIOGEN IDEC MA INC. - Cambridge MA

International Classification:

C12Q 1/02

US Classification:

435 29

Abstract:

Biomarkers useful for identifying treatments for and monitoring treatment of patients with multiple sclerosis (MS) are provided, as well as methods for their identification, methods of diagnosing MS, relapse of MS patients and disease progression in MS patients.

US Patent:

20120013580, Jan 19, 2012

Filed:

Jul 15, 2011

Appl. No.:

13/183676

Inventors:

Hua LIN - Fremont CA, US
Xudong Mike CUI - Fremont CA, US

International Classification:

G09G 5/00

US Classification:

345204

Abstract:

Systems, methods, and other embodiments associated with controlling pixels of a display are described. According to one embodiment, an apparatus includes a frame counter logic configured to maintain a global frame counter to track a number of refreshes of a display while updating pixels. The frame counter logic is also configured to determine a frame number when updating a pixel. The apparatus also includes a pixel logic configured to update the pixel.

US Patent:

61040282, Aug 15, 2000

Filed:

May 29, 1998

Appl. No.:

9/086993

Inventors:

Joanna M. Hunter - Redwood City CA
Hua Lin - Alameda CA
Christopher H. Becker - Palo Alto CA

Assignee:

GeneTrace Systems Inc. - Alameda CA

International Classification:

H01J 4404

US Classification:

250288

Abstract:

A sample preparation method is disclosed for volatilization and mass spectrometric analysis of nonvolatile high molecular weight molecules. Photoabsorbing molecules having significant sublimation rates at room temperature under vacuum, and preferably containing hydroxy functionalities, are disclosed for use as matrices in matrix-assisted laser desorption/ionization mass spectrometry. The samples are typically cooled in the mass spectrometer to temperatures significantly below room temperature.