Jason E Bell

US Patent:

20210372998, Dec 2, 2021

Filed:

Mar 22, 2021

Appl. No.:

17/209045

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

US Patent:

20210364502, Nov 25, 2021

Filed:

Aug 3, 2021

Appl. No.:

17/393107

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Jerald Sapida - San Jose CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

US Patent:

20210102936, Apr 8, 2021

Filed:

Jun 9, 2020

Appl. No.:

16/897126

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Berkeley CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/532
C07K 14/74
C12N 15/85
G01N 33/543
G01N 33/548
C12N 15/10
C12Q 1/6804
C12Q 1/6806
G01N 33/50
G01N 33/53
G01N 33/58
C12Q 1/6818
C12Q 1/6827
C12Q 1/6881
C12N 15/11
G01N 33/569

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

US Patent:

20200291454, Sep 17, 2020

Filed:

Feb 12, 2020

Appl. No.:

16/789287

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Jerald Sapida - San Jose CA, US

International Classification:

C12Q 1/6806
C12N 15/10
C12Q 1/6869
C12Q 1/6844

Abstract:

The present disclosure provides methods, systems, and kits for processing nucleic acid molecules. A method may comprise providing a template nucleic acid fragment (e.g., within a cell, cell bead, or cell nucleus) within a partition (e.g., a droplet or well) and subjecting the template nucleic acid fragment to one or more processes including a barcoding process and a single primer extension or amplification process. The processed template nucleic acid fragment may then be recovered from the partition and subjected to further amplification to provide material for subsequent sequencing analysis. The methods provided herein may permit simultaneous processing and analysis of both DNA and RNA molecules originating from the same cell, cell bead, or cell nucleus.

US Patent:

20200056223, Feb 20, 2020

Filed:

Jun 6, 2019

Appl. No.:

16/434095

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US

International Classification:

C12Q 1/6806

Abstract:

Provided herein are compositions and methods for cellular analysis. Nucleic acid from single cells may be processed in one or more partitions. A partition may comprise a cell bead and one or more enzymes for nucleic acid processing. A partition may comprise a functionalized polymer. In some cases, single cells may be subjected to epigenetic analysis, thereby generating an epigenetic profile for each cell from a plurality of cells.

US Patent:

20190383798, Dec 19, 2019

Filed:

Apr 4, 2019

Appl. No.:

16/375110

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/50
G01N 33/543
G01N 33/53
G01N 33/569
C12N 15/10

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and antigen screening. Polynucleotide processing may be useful for a variety of applications. Antigen screening may comprise the use of one or more engineered cells. Engineered cells may be useful for characterizing one or more analytes including, for example, a polypeptide antigen.

US Patent:

20190383804, Dec 19, 2019

Filed:

Apr 4, 2019

Appl. No.:

16/375119

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

G01N 33/53
G01N 33/543
C12N 15/11

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

US Patent:

20190367966, Dec 5, 2019

Filed:

Apr 4, 2019

Appl. No.:

16/375102

Inventors:

- Pleasanton CA, US
Jason Bell - Palo Alto CA, US
Zachary Bent - Pleasanton CA, US
Rajiv Bharadwaj - Pleasanton CA, US
Christopher Hindson - Livermore CA, US
Bill Kengli Lin - Pleasanton CA, US
Anthony Makarewicz - Livermore CA, US
Geoffrey McDermott - Livermore CA, US
Elliott Meer - Pleasanton CA, US
Francesca Meschi - Menlo Park CA, US
Tarjei Sigurd Mikkelsen - Dublin CA, US
Christopher Joachim O'Keeffe - Pleasanton CA, US
Katherine Pfeiffer - Pleasanton CA, US
Andrew D. Price - Hayward CA, US
Paul Ryvkin - San Jose CA, US
Sarah Taylor - Fremont CA, US
Jessica Michele Terry - Pleasanton CA, US
Tobias Daniel Wheeler - Alameda CA, US
Yifeng Yin - Elk Grove CA, US
Xinying Zheng - San Jose CA, US
Solongo Batjargal Ziraldo - San Ramon CA, US
Eswar Prasad Ramachandran Iyer - Sunnyvale CA, US
Luigi Jhon Alvarado Martinez - Pleasanton CA, US

International Classification:

C12Q 1/6806
G01N 33/53
G01N 33/58
C12Q 1/6804
G01N 33/50
C12N 15/10

Abstract:

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.