Jeffery Joseph Ambrose

Laboratory Manager

view source

Location:

1704 Roosevelt Dr, Ruston, LA 71270

Industry:

Biotechnology

Work:

Eurofins
Laboratory Manager
Louisiana Tech University Sep 2009 - May 2016
Graduate Research Assistant
Joseph Douglass and Associates Sep 2009 - May 2016
Owner and Manager
Brookhaven National Laboratory Jun 2008 - Aug 2008
Internship

Education:

Louisiana Tech University 2009 - 2016
Doctorates, Doctor of Philosophy, Biomedical Engineering, Bioengineering, Philosophy Louisiana Tech University 2013
Southern University at New Orleans 2008 - 2009
Bachelors, Biology Hampton University 2008
Benjamin Franklin High School 2004

Skills:

Cell Culture
Pcr
Qpcr
Rt Pcr
Plasmid Isolation
Structural Genomics
Western Blotting
Southern Blot
Genetics
Bacterial Culture
Bacterial Transformation
Elisa
Rna Isolation
Transfection
Nanotechnology

Jeffery Ambrose

view source

Jeffery Ambrose New Orleans, LA

view source

Work:

Education:

Louisiana Tech University
Ruston, LA
2009 to 2000
Ph.D. in Biomedical Engineering Southern University
New Orleans, LA
2005 to 2009
B.Sc. in Biology

Jeffery Ambrose Ruston, LA

view source

Work:

General Chemistry Lab - LA Tech University

2012 to 2000
Teaching Assistant and Tutor Louisiana Tech University

2009 to 2000
Graduate Assistant Brookhaven National Laboratory
Upton, NY
Jun 2009 to Aug 2009
Research Intern Brookhaven National Laboratory
Upton, NY
Jun 2008 to Aug 2008
Research Intern

Education:

Louisiana Tech University
Ruston, LA
2009 to 2000
Ph.D. in Biomedical Engineering Southern University
New Orleans, LA
2008 to 2009
B.Sc. in Biology

Skills:

SKILLS<br/>GENERAL LABORATORY<br/> Pipette, weigh, sterilize, filtrate, titrate, wash/clean glassware, sterile techniques, pH and temperature measurements.<br/><br/>IN-VITR... AND EX-VIVO EXPERIMENTS<br/> Cell/tissue culture, preparation and sterilization of media, fluorescent staining, cytometry,<br/>cytotoxicity assays, incubators, measurement of IC50 and LD50 of biological compounds.<br/><br/>ANIMAL CARE<br/> Handle and restrain, weigh animals, injections, anesthetization, evaluate animal records,<br/>sterile surgical procedures, collect blood and other biological samples.<br/><br/>GENETIC ENGINEERING<br/> Gene transfection, Southern blot and Western blot, PCR, RT-PCR, Q-PCR, plasmid design,<br/>plasmid purification, gene expression, digestion, ligation, bacterial transformation, gene<br/>integration, tdl in system for integration, recombinase mediated cassette exchange (rmce),<br/>structural genomics, DNA shuffling, protein evolution.<br/><br/>ORGANIC MEDICINAL CHEMISTRY<br/> Fabrication of polymeric nanoparticles by emulsification-solvent evaporation, ionic gelation and coacervation methods.<br/><br/> Fabrication of liposomal and micellar nanoparticles using bangham and reverse phase<br/>evaporation methods.<br/><br/> Fabrication of multi-layer hybrid nanoparticles using lipid film hydration and emulsion<br/>methods for sequential drug delivery.<br/><br/> Surface functionalization of drug and gene loaded nanoparticles for improved<br/>pharmacokinetics and pharmacodynamics.<br/><br/> Determination of synergistic dosages for combinatorial therapy, measurement of encapsulation efficiency, drug loading and nanoparticle yield, modification of release<br/>patterns.<br/><br/... Light microscope, multiphoton fluorescence, microscopy, SEM, TEM, DLS, HPLC, UV/VIS,<br/>spectrophotometry, mechanical testing, pore size analysis, contact angle, thermal<br/>analysis(TGA), standard and high speed centrifuges, precision balance, NSLS beamline,<br/>fluorometer, microplate meter.<br/><br/>SOFTWARE AND COMPUTATIONAL ANALYSIS<br/> Microsoft Office<br/> Data Processing<br/> TA Instruments Universal Analysis (winUA)<br/> Image J