John W Backus

US Patent:

6475729, Nov 5, 2002

Filed:

Oct 29, 1999

Appl. No.:

09/430256

Inventors:

John Bruce Findley - Rochester NY
John Wesley Backus - Williamson NY
William Harold Donish - Rochester NY
John William H. Sutherland - Rochester NY

Assignee:

Johnson Johnson Clinical Diagnostics, Inc. - Rochester NY

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure. This procedure includes a series of steps which have critically defined temperature and time parameters. Each polymerase chain reaction cycle requires generally less than about two minutes, and in most cases less than 90 seconds. At least 5 units/100 l of solution of thermostable DNA polymerase are used, and other preferred levels of primer concentrations facilitate the quick cycling in the amplification. In preferred embodiments, only two temperatures are used in the amplification.

US Patent:

6699986, Mar 2, 2004

Filed:

Jul 9, 1999

Appl. No.:

09/349387

Inventors:

Carol Ann Kreader - Kirkwood MO
John Wesley Backus - Ballwin MO

Assignee:

Ortho-Clinical Diagnostics, Inc. - Rochester NY

International Classification:

C07H 2100

US Classification:

536 254, 204462, 435 6, 435 912

Abstract:

The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins found in the same biological sample by applying the sample to an electrophesis gel and subjecting the sample to electrophoresis under acid conditions to separate the nucleic acids from the proteins. The optimum pH may differ for different sample types but can be readily determined by those skilled in the art. Preferably, the separation is performed at a pH of about 2 to about 4. More preferably, electrophoresis is carried out at a pH of 2. 5.

US Patent:

8148157, Apr 3, 2012

Filed:

Jan 7, 2009

Appl. No.:

12/349695

Inventors:

John W. Backus - Ontario NY, US
Jian Zheng - Raritan NJ, US
George Bashirians - Penfield NY, US

Assignee:

Ortho-Clinical Diagnostics, Inc. - Raritan NJ

International Classification:

G01N 31/00

US Classification:

436 15, 435 15

Abstract:

Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

US Patent:

7267950, Sep 11, 2007

Filed:

May 1, 2003

Appl. No.:

10/427217

Inventors:

Robert Belly - Webster NY, US
Jacqueline Toner - Penfield NY, US
John Backus - Pittsford NY, US

Assignee:

Veridex, LCC - Raritan NJ

International Classification:

C12Q 1/68

US Classification:

435 6

Abstract:

A method of extracting RNA from biological systems (cells, cell fragments, organelles, tissues, organs, or organisms) is presented in which a solution containing RNA is contacted with a substrate to which RNA can bind. RNA is withdrawn from the substrate by applying negative pressure. No centrifugation step exceeds thirty seconds. Preferably, the RNA is diluted prior to filtration and one or more wash steps can be used to remove interferents. In one such method, except for DNA shearing and drying steps, centrifugation is not undertaken during the extraction step.

US Patent:

20070037186, Feb 15, 2007

Filed:

May 16, 2006

Appl. No.:

11/435266

Inventors:

Yuqiu Jiang - San Diego CA, US
John Backus - Ontario NY, US
Abhijit Mazumder - Basking Ridge NJ, US
Dondapati Chowdary - Princeton Junction NJ, US
Fei Yang - Eugene OR, US
Yixin Wang - San Diego CA, US
Timothy Jatkoe - San Diego CA, US

International Classification:

C12Q 1/68
G01N 33/574

US Classification:

435006000, 435007230

Abstract:

The present invention relates to methods, compositions and articles directed to diagnosing thyroid carcinoma, differentiating between thyroid carcinoma and benign thyroid diseases, testing indeterminate thyroid fine needle aspirate samples of thyroid nodules, and determining patient protocols and outcomes.

US Patent:

20110275079, Nov 10, 2011

Filed:

Nov 13, 2008

Appl. No.:

12/742920

Inventors:

John F. Palma - Carlsbad CA, US
John W. Backus - Ontario NY, US
Yixin Wang - Basking Ridge NJ, US
Jack X. Yu - San Diego CA, US
Yi Zhang - San Diego CA, US
Tatiana Vener - Stirling NJ, US
Carlo Derecho - Lakehurst NJ, US
Dong U. Lee - San Diego CA, US

International Classification:

C12Q 1/68
G01N 33/53

US Classification:

435 612, 436501

Abstract:

Methods are disclosed for the identification of gene sets that are differentially expressed in PBMCs of patients diagnosed with a pre-diabetic disease state and overt type II diabetes. 3 gene and 10 gene signatures are shown to accurately predict a diabetic disease state in a patient. The application also described kits for the rapid diagnosis of diabetic disease states in patients at a point of care facility.

US Patent:

20120142035, Jun 7, 2012

Filed:

Feb 21, 2012

Appl. No.:

13/401263

Inventors:

John W. Backus - Ontario NY, US
Jian Zheng - Raritan NJ, US
George Bashirians - Penfield NY, US

International Classification:

G01N 33/53

US Classification:

435 792

Abstract:

Disclosed herein are compositions and methods comprising two or more proteins in which at least one of the proteins has been altered to reduce their mutual recognition and binding. Such compositions are useful as reference, calibrators or controls in methods and assays for determining the amount of one or more of the proteins that may be present in a sample of interest or in confirming the presence of one or more of the proteins in the sample. More particularly, it relates to compositions and methods comprising altered placental growth factor-1 (PlGF-1) and soluble fms-like tyrosine kinase (sFlt-1) and methods for determining the amount or confirming the presence of sFlt-1 and/or PlGF-1 in a sample of interest.

US Patent:

20130217011, Aug 22, 2013

Filed:

Dec 4, 2012

Appl. No.:

13/693306

Inventors:

John W. Backus - Ontario NY, US
Yixin Wang - Basking Ridge NJ, US
Jack X. Yu - San Diego CA, US
Yi Zhang - San Diego CA, US
Tatiana Vener - Stirling NJ, US
Dong U. Lee - San Diego CA, US

Assignee:

Veridex, LLC - New Brunswick NJ

International Classification:

C12Q 1/68
G01N 33/68

US Classification:

435 611, 435 612, 435 71, 435 721, 702 20

Abstract:

Methods are disclosed for the identification of gene sets that are differentially expressed in PBMCs of patients diagnosed with a pre-diabetic disease state and overt type II diabetes. 3 gene and 10 gene signatures are shown to accurately predict a diabetic disease state in a patient. The application also described kits for the rapid diagnosis of diabetic disease states in patients at a point of care facility.