John A Yoder

US Patent:

40396525, Aug 2, 1977

Filed:

Oct 11, 1973

Appl. No.:

5/405316

Inventors:

Ernest Clarence Adams - Elkhart IN
Joe William Davis - Elkhart IN
John Menley Yoder - Elkhart IN

Assignee:

Miles Laboratories, Inc. - Elkhart IN

International Classification:

G01N 3300
G01N 3316
G21H 502

US Classification:

424 1

Abstract:

An improved method is disclosed for determining substances in a fluid sample having a mutual specific binding affinity, such as antigens and antibodies, said method utilizing a device comprising a column containing an insoluble porous matrix with which specific binding partners to the substance being determined are immobilized, preferably by chemically coupling them to the matrix material. The method comprises allowing a fluid sample containing the substance to be determined, a reference sample containing a labeled form of either the substance under determination or a specific binding partner, and an eluting liquid to flow through the device, followed by a determination of the relative amount of the labeled component retained in or eluted from the column. Comparison to standard values affords a reading of the concentration or absolute amount of the substance being determined in the fluid sample.