Kevin M Gardner

US Patent:

7132278, Nov 7, 2006

Filed:

Nov 19, 2001

Appl. No.:

10/059962

Inventors:

Steven L. McKnight - Dallas TX, US
Kevin Gardner - Dallas TX, US
Shannon Harper - Dallas TX, US
Jared Rutter - Dallas TX, US
Carolyn Michnoff - Dallas TX, US
Carlos Amezcua - Dallas TX, US

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C07H 21/04
C12N 5/00
C12N 1/16
C12N 15/00

US Classification:

435325, 43525411, 435194, 4353201, 536 235, 536 232, 536 231

Abstract:

The invention provides methods and compositions relating to a novel kinase designated PAS Kinase (PASK). The compositions include isolated polypeptides comprising a native PASK protein or a PASK N-terminal domain and polypeptides consisting of a PASK PAS-A or PAS-B domain, as well as isolated polynucleotides encoding such polypeptides, and expression vectors and cells comprising such polynucleotides. The methods include binding assays comprising the steps of incubating a mixture comprising a subject polypeptide with a ligand under conditions wherein the polypeptide binds the ligand; and detecting binding of the polypeptide to the ligand.

US Patent:

7645569, Jan 12, 2010

Filed:

Oct 1, 2003

Appl. No.:

10/677733

Inventors:

Kevin H. Gardner - Dallas TX, US
Carlos A. Amezcua - Dallas TX, US
Paulus J. A. Erbel - Dallas TX, US
Paul B. Card - Dallas TX, US

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C12Q 1/00
G01N 33/53

US Classification:

435 4, 435 71

Abstract:

Specific binding of a foreign core ligand to a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is determined using NMR spectra of the PAS domain in the presence and absence of a foreign ligand bound within the hydrophobic core. A functional surface binding specificity of a PAS domain is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.

US Patent:

20050074846, Apr 7, 2005

Filed:

Oct 1, 2003

Appl. No.:

10/677734

Inventors:

Kevin Gardner - Dallas TX, US
Carlos Amezcua - Dallas TX, US
Paulus Erbel - Dallas TX, US
Paul Card - Dallas TX, US
Shannon Harper - Dallas TX, US
Jared Rutter - Salt Lake City UT, US
Richard Bruick - Dallas TX, US
Steven McKnight - Dallas TX, US

International Classification:

C07H021/04
C12N009/12

US Classification:

435069100, 435320100, 435194000, 435325000, 536023200

Abstract:

Specific binding of a foreign core ligand to a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is determined by (a) detecting a first NMR spectrum of the PAS domain in the presence of a foreign ligand; and (b) comparing the first NMR spectrum with a second NMR spectrum of the PAS domain in the absence of the ligand to infer the presence the ligand specifically bound within the hydrophobic core of the PAS domain. A functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.

US Patent:

20060051829, Mar 9, 2006

Filed:

Oct 11, 2005

Appl. No.:

11/245742

Inventors:

Kevin Gardner - Dallas TX, US
Carlos Amezcua - Dallas TX, US
Paulus Erbel - Dallas TX, US
Paul Card - Dallas TX, US
Shannon Harper - Dallas TX, US
Jared Rutter - Salt Lake City UT, US
Richard Bruick - Dallas TX, US
Steven McKnight - Dallas TX, US

International Classification:

C12Q 1/48
C12N 9/12

US Classification:

435015000, 435194000

Abstract:

A functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.

US Patent:

63196794, Nov 20, 2001

Filed:

Jan 26, 2001

Appl. No.:

9/770170

Inventors:

Steven L. McKnight - Dallas TX
Kevin Gardner - Dallas TX
Shannon Harper - Dallas TX
Jared Rutter - Dallas TX
Carolyn Michnoff - Dallas TX
Carlos Amezcua - Dallas TX

Assignee:

Board of Regents, The University of Texas System - Austin TX

International Classification:

C12Q 148

US Classification:

435 15

Abstract:

The invention provides methods and compositions relating to a novel kinase designated PAS Kinase (PASK). The compositions include isolated polypeptides comprising a native PASK protein or a PASK N-terminal domain and polypeptides consisting of a PASK PAS-A or PAS-B domain, as well as isolated polynucleotides encoding such polypeptides, and expression vectors and cells comprising such polynucleotides. The methods include binding assays comprising the steps of incubating a mixture comprising a subject polypeptide with a ligand under conditions wherein the polypeptide binds the ligand; and detecting binding of the polypeptide to the ligand.

US Patent:

20170204421, Jul 20, 2017

Filed:

Nov 1, 2016

Appl. No.:

15/340040

Inventors:

Kevin H. Gardner - Dallas TX, US
Brian D. Zotowski - Dallas TX, US

International Classification:

C12N 15/63
C12N 15/62
C12N 9/02

Abstract:

The present invention provides methods for light-dependent gene regulation using a light-responsive DNA-binding protein. Also provided are related nucleic acid molecules, and protein molecules, such as those encoding or comprising the light-responsive DNA-binding protein or DNA-binding sites recognizing the light-responsive DNA-binding protein. Kits using the present light-dependent gene regulation system are further provided by the present invention.

US Patent:

20160250216, Sep 1, 2016

Filed:

Nov 14, 2013

Appl. No.:

14/442485

Inventors:

- Austin TX, US
Charles G. CALDWELL - Dallas TX, US
Doug E. FRANTZ - Boerne TX, US
Kevin H. GARDNER - Carrollton TX, US
John B. MACMILLAN - Dallas TX, US
Thomas H. SCHEUERMANN - Richardson TX, US
Uttam K. TAMBAR - Dallas TX, US

Assignee:

tHE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM - Austin TX

International Classification:

A61K 31/519
A61K 31/4184
A61K 31/4192
A61K 31/4245
A61K 31/136

Abstract:

Provided is a method of inhibiting heterodimerization of HIF-2α to HIF1β (ARNT) comprising binding certain small molecules to the HIF-2α PAS-B domain cavity but not to HIF1α and inhibiting HIF-2α heterodimerization to HIF1β (ARNT) but not inhibiting HIF1α heterodimerization to HIF1β (ARNT). Those certain small molecules are also referenced synonymously as HIF2-HDI and HIF2α heterodimerization inhibitors and also simply as certain small molecules.