Kimberly Lane Kelly

US Patent:

7452965, Nov 18, 2008

Filed:

Apr 7, 2003

Appl. No.:

10/510155

Inventors:

Kimberly A. Kelly - Burlington MA, US
David A. Jones - Salt Lake City UT, US

Assignee:

University of Utah Research Foundation - Salt Lake City UT

International Classification:

A61K 38/12

US Classification:

530317, 514 12

Abstract:

Phage display was used to screen peptide libraries that distinguish between well-differentiated (HCT116) and poorly-differentiated colon carcinoma cells (HT29). The screening protocol used selection and subtraction on intact, viable cells, resulting in phage libraries exhibiting high binding selectivity for the poorly-differentiated HT29 cells. A nine amino acid, disulfide-constrained peptide (RPM) was identified that selectively bound and was internalized into colon cancer cells. The peptide may be used to detect colon cancer cells and also may be used to selectively deliver therapeutic agents to the cells.

US Patent:

20100233676, Sep 16, 2010

Filed:

Jun 12, 2008

Appl. No.:

12/663370

Inventors:

Kimberly Kelly - Burlington MA, US
Ralph Weissleder - Peabody MA, US

International Classification:

C12Q 1/04
C07K 5/10
C07K 7/06
C07K 7/08
C07K 14/00
C12Q 1/70
C12N 15/63
C12N 15/85
C12N 15/74
C12N 15/82

US Classification:

435 5, 530330, 530329, 530328, 530327, 530326, 530402, 435 34, 435440, 435455, 435471, 435468

Abstract:

The present invention contemplates strategies comprising small molecule, cell permeable probes that allow site-specific protein labeling for visualizing biological processes. In one embodiment, the present invention contemplates a series of short peptide sequences comprising high affinity binding (i.e., for example, subnanomolar affinity (0.53 nM) for indocyanine fluorochromes. In one embodiment, the peptide sequences comprise a 5 pmol detection limit for indocyanine fluorochromes. In one embodiment, the present invention contemplates methods comprising high affinity peptide-fluorochrome binding pairs in biological applications including, but not limited to, enzyme linked immunoabsorbent assay (ELISA), fluorescence activated cell sorting (FACS), microscopy (i.e., for example scanning electromicroscopy), Western Blots, histochemistry, protein and cell based tracking both in vitro and in vivo.