Lee R Moore

US Patent:

6412359, Jul 2, 2002

Filed:

Jun 30, 2000

Appl. No.:

09/607702

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee Robert Moore - Cleveland Heights OH

Assignee:

The Cleveland Clinc Foundation - Cleveland OH
The Ohio State University - Columbus OH

International Classification:

G01N 2100

US Classification:

738655

Abstract:

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle.

US Patent:

6557430, May 6, 2003

Filed:

Jul 1, 2002

Appl. No.:

10/187457

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee Robert Moore - Cleveland Heights OH

Assignee:

The Cleveland Clinic Foundation - Cleveland OH
The Ohio State University - Columbus OH

International Classification:

G01N 2100

US Classification:

738655, 335297

Abstract:

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle.

US Patent:

20100093052, Apr 15, 2010

Filed:

Nov 14, 2007

Appl. No.:

12/514618

Inventors:

Jeffrey J. Chalmers - Columbus OH, US
Xiaodong Tong - Columbus OH, US
Maciej Zborowski - Bay Village OH, US
Lee R. Moore - Sagamore Hills OH, US
Sherif S. Farag - Carmel IN, US

International Classification:

C12N 13/00
C12M 1/42

US Classification:

4351739, 4353081

Abstract:

Systems and methods are provided separating a first group of cells from a mixture of at least first and second groups of cells in a suspending fluid. The system includes an annular flow channel and at least one magnetic element positioned around the exterior of the annular flow channel as to provide a radially symmetric magnetic field around at least a portion of the annular flow channel. The at least one magnetic element is configured to provide a gradually increasing magnetic field along the axis of flow as to limit the maximum magnetic gradient within the annular flow channel. A pump is operatively connected to a terminal end of the annular flow channel to force the suspending fluid through the annular channel.

US Patent:

20130315796, Nov 28, 2013

Filed:

Feb 17, 2011

Appl. No.:

13/758695

Inventors:

Jeffrey J. Chalmers - Columbus OH, US
Maciej Zborowski - Bay Village OH, US
Thomas J. Ward - Columbus OH, US
George Keller - Columbus OH, US
Adam Landis - Reynoldsburg OH, US
Michael Ward - Columbus OH, US
Lee Robert Moore - Sagamore Hills OH, US

Assignee:

THE OHIO STATE UNIVERSITY - Columbus OH

International Classification:

G01N 33/487

US Classification:

422513

Abstract:

A disposable kit for use in directing fluid through a biological cell separator device (). The kit generally includes a separator tube (), a buffer fluid container (), cell sample container (), separated cell container (), and flushing fluid container (), as well as various conduits () for connecting the containers () and separator tube () in fluid communication together. A cell separator system is provided including a separator tube (), magnet (), pump () and a motorized drive unit (). The motorized drive unit () is operatively connected to the magnet () to allow the magnet () to be moved a sufficient distance away from the separator tube () so as to allow cells adhered to the inside surface thereof to be flushed out of the tube ().

US Patent:

61420255, Nov 7, 2000

Filed:

Jan 19, 1999

Appl. No.:

9/232956

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee Robert Moore - Cleveland Heights OH

Assignee:

The Cleveland Clinic Foundation - Cleveland OH

International Classification:

G01N 2100

US Classification:

738655

Abstract:

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle.

US Patent:

60822058, Jul 4, 2000

Filed:

Feb 6, 1998

Appl. No.:

9/020327

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee Robert Moore - Cleveland Heights OH

Assignee:

Ohio State University - Columbus OH
The Cleveland Clinic Foundation - Cleveland OH

International Classification:

G01N 2100

US Classification:

738655

Abstract:

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle.

US Patent:

59688203, Oct 19, 1999

Filed:

Feb 26, 1997

Appl. No.:

8/806598

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee R. Moore - Cleveland Heights OH

Assignee:

The Cleveland Clinic Foundation - Cleveland OH
The Ohio State University - Columbus OH

International Classification:

C12N 500
C12N 1300
A01N 102
B03C 100
B07C 502
B07C 900
B01D 3506

US Classification:

435325

Abstract:

A method for separating cells is presented. The method provides a flow through fractional cell sorting process that is based on the application of a magnetic force to cells having a range of magnetic labeling densities. Magnetically labeled cells are introduced into a flow channel. The cells are continuously fractionated into flow streams based on magnetic susceptibility by subjecting the cells in the flow channel to a magnetic field. The fractionated cells are recovered based on their response to the magnetic field. Methods employing dipole fractional sorters and quadrapole annular sorters are also presented.

US Patent:

61207357, Sep 19, 2000

Filed:

Jul 13, 1999

Appl. No.:

9/352725

Inventors:

Maciej Zborowski - Bay Village OH
Jeff Chalmers - Columbus OH
Lee R. Moore - Cleveland Heights OH

Assignee:

The Ohio States University - Columbus OH
The Cleveland Clinic Foundation - Cleveland OH

International Classification:

G01N 3300

US Classification:

422 73

Abstract:

A method and apparatus for the fraction sorting of cells is provided. The invention include a dispensing device, aspirating device, flow channel and a magnetic assembly. A first fractional sorting device includes a dipole fractional cell sorter. The dipole fractional cell sorter includes large aspect ratio flow channel and a dipole magnetic assembly. A second fractional sorting device includes a quadrupole fractional cell sorter. The quadrupole fractional cell sorter includes an annular flow channel having inlet and outlet splitters, and a quadrupole magnetic assembly. The quadrupole magnetic assembly includes pole pieces having projecting members with convex surfaces and in is in physical communication with the annular flow chamber. The method of the present invention provides a flow through fractional cell sorting process which is based on the application of a magnetic force to cells having a range of magnetic labeling densities.