Chuan He - Chicago IL, US Liang Zhang - Chicago IL, US Chunxiao Song - Chicago IL, US
Assignee:
THE UNIVERSITY OF CHICAGO, UCHICAGO TECH - Chicago IL
International Classification:
C12Q 1/68 C40B 30/00
US Classification:
506 7, 435 611
Abstract:
The present invention relates generally to the field of molecular biology. More particularly, it concerns methods and compositions for detecting, evaluating, and/or mapping 5-hydroxymethyl-modified cytosine bases within a nucleic acid molecule.
Identification Of 5-Methyl-C In Nucleic Acid Templates
Tyson A. Clark - Menlo Park CA, US Liang Zhang - Chicago IL, US Xingyu Lu - Chicago IL, US
Assignee:
University of Chicago - Chicago IL Pacific Biosciences of California, Inc. - Menlo Park CA
International Classification:
C12Q 1/68
US Classification:
435 611
Abstract:
A method for identifying a 5-MeC in a template nucleic is provided. The method comprises providing a template having 5-MeC, converting the 5-MeC into a futher modification selected from 5-caC and 5-FC. The converted template is then sequenced, and a change in sequencing is detected that is indicative of the further modification, allowing for identifying the 5-MeC in the template nucleic acid.
Composition And Methods Related To Modification Of 5-Methylcytosine (5-Mc)
Chuan He - Chicago IL, US Liang Zhang - Chicago IL, US Xingyu Lu - Chicago IL, US
Assignee:
The University of Chicago - Chicago IL
International Classification:
C12Q 1/68
US Classification:
435 611
Abstract:
The present invention relates to methods and compositions for detecting, evaluating, and/or mapping 5-methyl-modified and/or 5-hydroxylmethyl-modified cytosine bases within a nucleic acid molecule.