Linh B Phan

US Patent:

57445877, Apr 28, 1998

Filed:

Jun 7, 1995

Appl. No.:

8/484246

Inventors:

Andrew R. Alaska - Issaquah WA
William Downey - Seattle WA
John W. Forstrom - Seattle WA
Linh Phan - Seattle WA

Assignee:

ZymoGenetics, Inc. - Seattle WA

International Classification:

A61K 3824
A61K 3827
C04K 100
C04K 1400

US Classification:

530399

Abstract:

Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.