Luis A Baez

US Patent:

20020051986, May 2, 2002

Filed:

May 16, 2001

Appl. No.:

09/858994

Inventors:

Luis Baez - West Chester PA, US
Richard Ebersole - Newark DE, US
Edwin Hendrickson - Hockessin DE, US
Neel Neelkantan - Newark DE, US
Michael Perry - Downington PA, US

International Classification:

C12Q001/68
G01N033/53
C12P019/34
G01N033/543

US Classification:

435/006000, 435/007500, 436/518000, 435/091100

Abstract:

A process is disclosed for the detection of an analyte utilizing a nucleic acid label as a reporter. The analyte is detected by the binding of at least two reporter conjugates, each conjugate comprising a member of a binding pair and a nucleic acid label. The binding of the reporter conjugates to the analyte facilitates the juxtaposition of the nucleic acid labels, forming a single nucleic acid amplicon. The amplicon may then be detected directly, or may be used as a template of the generation of amplification products. Detection of the analyte by this process significantly reduces assay background caused by non-specific reporter conjugate binding.

US Patent:

20090197299, Aug 6, 2009

Filed:

Nov 29, 2006

Appl. No.:

12/085738

Inventors:

Diego Vargas - Lansdale PA, US
Luis A. Baez - Simi Valley CA, US

Assignee:

MERCK & CO., INC. - Rahway NJ

International Classification:

C12Q 1/06
C12Q 1/04

US Classification:

435 39, 435 34

Abstract:

The present invention relates to an environmental sampling method for assessing airborne microorganisms. The method comprises retaining organisms suspended in air to a polymeric pad via gravitational settling or forced impact, dissolving the polymeric pad in a buffered salt solution, and determining the number and kind of microorganisms in the buffered salt solution. Neither the polymeric pad nor the buffered salt solution inhibits the growth of the microorganisms to be determined.