Mathewos Z Tessema

US Patent:

7785900, Aug 31, 2010

Filed:

Jan 11, 2007

Appl. No.:

11/652432

Inventors:

Peter C. Simons - Albuquerque NM, US
Larry A. Sklar - Albuquerque NM, US
Eric R. Prossnitz - Albuquerque NM, US
Mathewos Z. Tessema - Albuquerque NM, US
John C. Reed - Ranch Santa Fe CA, US
Dayong Zhai - San Diego CA, US

Assignee:

STC.UNM - Albuquerque NM
Sanford-Burnham Medical Research Institute - La Jolla CA

International Classification:

G01N 33/543
G01N 33/53

US Classification:

436523, 436501, 436506, 436518, 436524, 436528, 435 71, 435 721, 422 50, 422 61, 424 91, 530300, 530350

Abstract:

The present invention relates generally to glutathione derivatized beads which are adapted for use in conjunction with glutathione-S-transferase fusion proteins (generally, GST fusion proteins, which contain a fluorescent label such as fluorescent green protein) for use in flow cytometry. The present invention also relates to methods for detecting and/or quantifying interactions between a GST fusion protein and their binding partners, in particular, labeled binding partners such as fluorescently labeled binding partners. By creating glutathione beads with an appropriate high or increased site density, disadvantages often associated with low affinity systems and quick off-rates in solution may be resolved to provide a workable system and method. Methods of identifying potential agonists, antagonists and regulator compounds of proteins fused to GST from libraries of compounds represents another aspect of the present invention.