Matthew Heckert

US Patent:

20120042412, Feb 16, 2012

Filed:

Aug 12, 2011

Appl. No.:

13/208960

Inventors:

Henrik Albert - Alameda CA, US
Linda A. Castle - Mountain View CA, US
Matthew Heckert - Union City CA, US
Jian Lu - Union City IA, US
Daniel L. Siehl - Menlo Park CA, US
Yumin Tao - Urbandale IA, US

Assignee:

Pioneer Hi-Bred International, Inc. - Johnston IA

International Classification:

A01H 1/06
C12N 15/82
C12N 5/10
C12N 9/96
A01H 5/10
C07K 19/00
C12N 9/02
C12N 15/62
A01H 5/00

US Classification:

800278, 536 234, 536 232, 4353201, 435419, 435412, 435416, 435415, 800298, 530350, 435189, 435418, 435188

Abstract:

Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

US Patent:

20120042413, Feb 16, 2012

Filed:

Aug 12, 2011

Appl. No.:

13/208966

Inventors:

Henrik Albert - Alameda CA, US
Ericka R. Bermudez - Aptos CA, US
Linda A. Castle - Mountain View CA, US
Yuxia Dong - San Ramon CA, US
Matthew J. Heckert - Union City CA, US
Jingtong Hou - San Pablo CA, US
Zhenglin Hou - Ankeny IA, US
Jian Lu - Union City CA, US
Daniel L. Siehl - Menlo Park CA, US
Yumin Tao - Urbandale IA, US

Assignee:

Pioneer Hi-Bred International, Inc. - Johnston IA

International Classification:

C12N 15/82
C12N 15/63
C12N 5/10
A01H 5/00
A01H 5/10
C12N 9/02
C12Q 1/68
A01N 41/10
A01N 43/80
A01N 43/56
A01N 57/20
A01N 43/90
A01N 43/54
A01P 13/00
G01N 33/573
C12N 15/53

US Classification:

800278, 536 232, 4353201, 435419, 435413, 435415, 435416, 800300, 435189, 435468, 435 618, 504348, 504271, 504282, 504128, 504130, 504136, 504138, 504139, 504118, 435 74, 435 612

Abstract:

Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.