Meredith H Jones

US Patent:

20230130038, Apr 27, 2023

Filed:

Oct 31, 2022

Appl. No.:

17/977788

Inventors:

- Carlsbad CA, US
Chao Yan LIU - Germantown MD, US
Jeffrey ROGERS - San Diego CA, US
Maria Isabel CISNEROS - San Diego CA, US
Jingqui LI - San Diego CA, US
Henry C. CHIOU - Encinitas CA, US
Meredith Bauman JONES - Chapel Hill NC, US

International Classification:

C07K 14/505
C12N 5/00
C12N 5/0793

Abstract:

High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

US Patent:

20190077841, Mar 14, 2019

Filed:

Jul 26, 2018

Appl. No.:

16/046641

Inventors:

- Carlsbad CA, US
Chao Yan LIU - Germantown MD, US
Jeffrey ROGERS - San Diego CA, US
Maria CISNEROS - San Diego CA, US
Jingqiu LI - San Diego CA, US
Henry CHIOU - Encinitas CA, US
Meredith JONES - Chapel Hill NC, US

International Classification:

C07K 14/505
C12N 5/0793
C12N 5/00

Abstract:

High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.