Michael J Mcclelland

US Patent:

6566053, May 20, 2003

Filed:

Dec 27, 1995

Appl. No.:

08/579445

Inventors:

Manuel Perucho - San Diego CA
Miguel Angel Peinado - Barcelona, ES
Yurij Ionov - San Diego CA
Sergei Malkhosyan - San Diego CA
Michael McClelland - Encinitas CA
John Welsh - Leucadia CA

Assignee:

Stratagene - La Jolla CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

US Patent:

6696277, Feb 24, 2004

Filed:

Mar 1, 1995

Appl. No.:

08/397335

Inventors:

Michael McClelland - Del Mar CA
John T. Welsh - Leucadia CA
Joseph A. Sorge - Rancho Santa Fe CA

Assignee:

Stratagene - La Jolla CA
California Institute of Biological Research - La Jolla CA

International Classification:

C12P 1934

US Classification:

435 912, 435 6, 435 911

Abstract:

A method for generating a discrete set of DNA segments characteristic of a sample of a single-stranded RNA is provided.

US Patent:

7090978, Aug 15, 2006

Filed:

Mar 20, 2003

Appl. No.:

10/394485

Inventors:

Manuel Perucho - San Diego CA, US
Miguel Angel Peinado - Barcelona, ES
Yurij Ionov - San Diego CA, US
Sergei Malkhosyan - San Diego CA, US
Michael McClelland - Encinitas CA, US
John Welsh - Leucadia CA, US

Assignee:

Stratagene California - La Jolla CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 2431, 536 2433

Abstract:

The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

US Patent:

7816080, Oct 19, 2010

Filed:

Nov 27, 2006

Appl. No.:

11/605049

Inventors:

Rhonda J. Honeycutt - Solana Beach CA, US
Michael McClelland - Encinitas CA, US

Assignee:

Clarity Biosciences, Inc. - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 243, 536 2433

Abstract:

The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.

US Patent:

20060292572, Dec 28, 2006

Filed:

Jan 10, 2005

Appl. No.:

11/033056

Inventors:

Robert Stuart - San Diego CA, US
Elizabeth Stuart - Sandiego, CA
William Wachsman - San Diego CA, US
Daniel Mercola - Rancho Santa Fe CA, US
Michael McClelland - Encinitas CA, US
David Tarin - San Diego CA, US
Charles Berry - San Diego CA, US
Karen Arden - San Diego CA, US
Linda Wasserman - San Diego CA, US
Steven Goodison - Jacksonville FL, US
Igor Klacansky - San Diego CA, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435006000, 435287200

Abstract:

Among the methods, compositions, combinations and kits provided herein are those for determining gene expression levels in one or more cell types in heterogeneous cell samples, for identifying genes differentially expressed in different cell types, and for detecting a cell type in a sample from a subject. Also provided herein are methods, compositions, combinations and kits for determining gene expression levels in cells corresponding to phenotypes, and for identifying a phenotype of a subject by detecting differentially expressed genes.

US Patent:

20070044660, Mar 1, 2007

Filed:

Aug 30, 2005

Appl. No.:

11/216422

Inventors:

Michael McClelland - Vancouver WA, US

International Classification:

B01D 46/00

US Classification:

095279000

Abstract:

An air filter dry cleaning system and method of dry cleaning an air filter is disclosed. The system includes a portable, mostly enclosed cabinet into which an air filter can be placed on two rollers. A blower hose attached to a blower motor blows diffused air onto the filter to dislodge particulate matter. A vacuum hose connected to a vacuum blower motor provides a diffused vacuum air flow to vacuum particulate matter from the air filter. A vacuum air flow connected to the same vacuum blower motor provides a vacuum air flow in the cabinet below the filter. The air vacuum air flow can be connected to a separate detachable dust collection system. Both the portable cabinet and dust collection system can be mounted on wheeled support structures.

US Patent:

62078100, Mar 27, 2001

Filed:

Nov 16, 1993

Appl. No.:

8/154364

Inventors:

Michael McClelland - Del Mar CA
John T. Welsh - Leucadia CA

Assignee:

Stratagene and California Institute of Biological Research - La Jolla CA

International Classification:

C07H21/00

US Classification:

536 231

Abstract:

A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a "fingerprint" comprises the steps of: priming target nucleic acid of a genome or from a cellular RNA preparation with an single-stranded primer to form primed nucleic acid such that a substantial degree of internal-mismatching occurs between the primer and the target nucleic acid; amplifying the primed nucleic acid by performing at least one cycle of polymerase chain reaction amplification; and amplifying the product of step (2) by performing at least about 10 cycles of polymerase chain reaction amplification. The method is known as the arbitrarily primed polymerase chain reaction (AP-PCR) method and is suitable for the identification of bacterial species and strains, mammals and plants. The method of the present invention can identify species, cell types or tissues rapidly, and does not require knowledge of the nucleotide sequence or other molecular biology of the nucleic acids of the organisms to be identified. The polynucleotide sequence LF9.

US Patent:

54379750, Aug 1, 1995

Filed:

Feb 25, 1991

Appl. No.:

7/661591

Inventors:

Michael McClelland - Del Mar CA
John T. Welsh - Leucadia CA

Assignee:

California Institute of Biological Research - La Jolla CA

International Classification:

C12Q 168
C12P 1934
C07H 2104

US Classification:

435 6

Abstract:

A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a "fingerprint" for typing the genome comprises the steps of: forming a polymerase chain reaction (PCR) admixture by combining, in a PCR buffer, genomic DNA and at least one structural RNA consensus primer, and subjecting the PCR admixture to a plurality of PCR thermocycles to produce a plurality of DNA segments, thereby forming a set of discrete DNA amplification products. The method is known as the consensus sequence primed polymerase chain reaction (CP-PCR) method and is suitable for the identification of bacterial species and strains, including Staphylococcus and Streptococcus species, mammals and plants. The method of the present invention can identify species rapidly, using only a small amount of biological material, and does not require knowledge of the nucleotide sequence or other molecular biology of the nucleic acids of the organisms to be identified. Only one primer sequence is required for amplification and/or identification.