Michael Mcclelland

US Patent:

6495319, Dec 17, 2002

Filed:

Apr 27, 1999

Appl. No.:

09/300958

Inventors:

Michael McClelland - Encinitas CA
John Welsh - Leucadia CA
Thomas Trenkle - San Diego CA

Assignee:

Sidney Kimmel Cancer Center - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 536 2432, 536 243, 536 231, 436504

Abstract:

The invention provides a method of measuring the level of two or more nucleic acid molecules in a target by contacting a probe with a target comprising two or more nucleic acid molecules, wherein the nucleic acid molecules are arbitrarily sampled and wherein the arbitrarily sampled nucleic acid molecules comprise a subset of the nucleic acid molecules in a population of nucleic acid molecules; and detecting the amount of specific binding of the target to the probe. The invention also provides a method of measuring the level of two or more nucleic acid molecules in a target by contacting a probe with a target comprising two or more nucleic acid molecules, wherein the nucleic acid molecules are statistically sampled and wherein the statistically sampled nucleic acid molecules comprise a subset of the nucleic acid molecules in a population of nucleic acid molecules; and detecting the amount of specific binding of the target to the probe.

US Patent:

6566053, May 20, 2003

Filed:

Dec 27, 1995

Appl. No.:

08/579445

Inventors:

Manuel Perucho - San Diego CA
Miguel Angel Peinado - Barcelona, ES
Yurij Ionov - San Diego CA
Sergei Malkhosyan - San Diego CA
Michael McClelland - Encinitas CA
John Welsh - Leucadia CA

Assignee:

Stratagene - La Jolla CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912

Abstract:

The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

US Patent:

6599701, Jul 29, 2003

Filed:

Aug 23, 2000

Appl. No.:

09/645055

Inventors:

Rhonda J. Honeycutt - Carlsbad CA
Michael McClelland - Encinitas CA

Assignee:

Clarity Biosciences, Inc. - San Diego CA

International Classification:

C12Q 168

US Classification:

435 6, 435 912, 536 2433, 536 243

Abstract:

The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.

US Patent:

6696277, Feb 24, 2004

Filed:

Mar 1, 1995

Appl. No.:

08/397335

Inventors:

Michael McClelland - Del Mar CA
John T. Welsh - Leucadia CA
Joseph A. Sorge - Rancho Santa Fe CA

Assignee:

Stratagene - La Jolla CA
California Institute of Biological Research - La Jolla CA

International Classification:

C12P 1934

US Classification:

435 912, 435 6, 435 911

Abstract:

A method for generating a discrete set of DNA segments characteristic of a sample of a single-stranded RNA is provided.

US Patent:

7090978, Aug 15, 2006

Filed:

Mar 20, 2003

Appl. No.:

10/394485

Inventors:

Manuel Perucho - San Diego CA, US
Miguel Angel Peinado - Barcelona, ES
Yurij Ionov - San Diego CA, US
Sergei Malkhosyan - San Diego CA, US
Michael McClelland - Encinitas CA, US
John Welsh - Leucadia CA, US

Assignee:

Stratagene California - La Jolla CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 2431, 536 2433

Abstract:

The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

US Patent:

7816080, Oct 19, 2010

Filed:

Nov 27, 2006

Appl. No.:

11/605049

Inventors:

Rhonda J. Honeycutt - Solana Beach CA, US
Michael McClelland - Encinitas CA, US

Assignee:

Clarity Biosciences, Inc. - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 6, 435 912, 536 243, 536 2433

Abstract:

The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.

US Patent:

20030224382, Dec 4, 2003

Filed:

Nov 7, 2002

Appl. No.:

10/291808

Inventors:

Michael McClelland - Encinitas CA, US
John Welsh - Leucadia CA, US
Thomas Trenkle - San Diego CA, US

Assignee:

Sidney Kimmel Cancer Center

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The invention provides a method of measuring the level of two or more nucleic acid molecules in a target by contacting a probe with a target comprising two or more nucleic acid molecules, wherein the nucleic acid molecules are arbitrarily sampled and wherein the arbitrarily sampled nucleic acid molecules comprise a subset of the nucleic acid molecules in a population of nucleic acid molecules; and detecting the amount of specific binding of the target to the probe. The invention also provides a method of measuring the level of two or more nucleic acid molecules in a target by contacting a probe with a target comprising two or more nucleic acid molecules, wherein the nucleic acid molecules are statistically sampled and wherein the statistically sampled nucleic acid molecules comprise a subset of the nucleic acid molecules in a population of nucleic acid molecules; and detecting the amount of specific binding of the target to the probe.

US Patent:

20040137463, Jul 15, 2004

Filed:

Jun 25, 2003

Appl. No.:

10/607559

Inventors:

Rhonda Honeycutt - Carlsbad CA, US
Michael McClelland - Encinitas CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.