Michael J Rice

US Patent:

6410226, Jun 25, 2002

Filed:

Sep 11, 1997

Appl. No.:

08/927165

Inventors:

Eric B. Kmiec - Yardley PA
William K. Holloman - Yorktown Heights NY
Michael C. Rice - Newtown PA
Sheryl T. Smith - Bryn Mar PA
Zhigang Shu - Bensalem PA

Assignee:

Thomas Jefferson University - Philadelphia PA
Cornell Research Foundation, Inc. - Ithaca NY

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 231, 536 243

Abstract:

The invention concerns mammalian recombinase genes (REC2) and their promoters. Over expression of REC2 in a cell is found to facilitate homologous recombination, particularly homologous recombination using a DNA/RNA chimeric oligonucleotide and to sensitize a cell to the apoptotic effects of irradiation. The REC2 promoter, in combination with a strong enhancer, e. g. , a SV40 enhancer, was found to be a strong promoter following irradiation of the cells. A radiation induceable promoter can be used to sensitize a cell to radiation treatment by operably linking the radiation induceable promoter to a gene whose expression converts a prodrug to a drug such as a herpes thymidien kinase gene.

US Patent:

7094614, Aug 22, 2006

Filed:

Jan 16, 2001

Appl. No.:

09/681126

Inventors:

Douglas S. Armbrust - Gloucester MA, US
John M. Baker - Yorktown Heights NY, US
Arne W. Ballantine - Round Lake NY, US
Roger W. Cheek - Essex Junction VT, US
Doreen D. DiMilia - Pleasantville NY, US
Mark L. Reath - St. Albans VT, US
Michael B. Rice - Colchester VT, US

Assignee:

International Business Machines Corporation - Armonk NY

International Classification:

H01L 21/66
B05C 11/00

US Classification:

438 14, 118712, 42725523

Abstract:

A method and apparatus are provided for controlling a CVD process used to deposit films on semiconductor substrates wherein the by-products of the reaction are measured and monitored during the reaction preferably using mass spectrometry and the results used to calculate the concentrations of the by-products and to control the CVD reaction process based on the by-product concentrations. An exemplary CVD process is the deposition of tungsten metal on a semiconductor wafer. A preferred method and apparatus uses a capillary gas sampling device for removing the by-product gases of the reaction as a feed for the mass spectrometer. The capillary gas sampling device is preferably connected to a differential pump.

US Patent:

61746940, Jan 16, 2001

Filed:

Sep 21, 1998

Appl. No.:

9/157603

Inventors:

Pamela A. Havre - Philadelphia PA
Michael C. Rice - Newtown PA
William K. Holloman - Yorktown Heights NY
Eric B. Kmiec - Yardley PA

Assignee:

Thomas Jefferson University - Philadelphia PA
Cornell Research Foundation, Inc. - Ithaca NY
Valigen (US), Inc. - Newtown PA

International Classification:

C12Q 148
C12N 912

US Classification:

435 15

Abstract:

The invention includes a method of phosphorylating a serine containing substrate by incubating the substrate with ATP and an enzyme that is hsRec2 or muRec2 or a derivative thereof. The natural substrates of the kinase activity of Rec2 are the cell cycle control proteins such as p53 and cyclin E. The over expression of Rec2 is known to cause cell-cycle arrest and apoptosis and the invention discloses that these effects are kinase mediated. Accordingly, the invention provides a method of assessing antagonists and agonists of Rec2, which antagonists and agonists would have pharmacological activity. The invention further discloses that there is specific binding between hsRec2 and at least three cell cycle control proteins: p53, PCNA and cdc2.

US Patent:

62109162, Apr 3, 2001

Filed:

Jun 5, 2000

Appl. No.:

9/587436

Inventors:

Pamela A. Havre - Philadelphia PA
Michael C. Rice - Newtown PA
William K. Holloman - Yorktown Heights NY
Eric B. Kmiec - Yardley PA

Assignee:

Thomas Jefferson University - Philadelphia PA
Cornell Research Foundation Inc. - Ithaca NY
Valigen (US), Inc. - Newtown PA

International Classification:

C12Q 148
C12N 912
C07K 100

US Classification:

435 15

Abstract:

The invention includes a method of phosphorylating a serine containing substrate by incubating the substrate with ATP and an enzyme that is hsRec2 or muRec2 or a derivative thereof. The natural substrates of the kinase activity of Rec2 are the cell cycle control proteins such as p53 and cyclin E. The over expression of Rec2 is known to cause cell-cycle arrest and apoptosis and the invention discloses that these effects are kinase mediated. Accordingly, the invention provides a method of assessing antagonists and agonists of Rec2, which antagonists and agonists would have pharmacological activity. The invention further discloses that there is specific binding between hsRec2 and at least three cell cycle control proteins: p53, PCNA and cdc2.